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JPT/PepMix™ HIV (NEF) Ultra/PM-HIV-NEF/

PepMix™HIV(NEF)Ultra

ProductCode:PM-HIV-NEF

Poolof15015mericpeptidesderivedfromNefproteindesignedtocoverthehighsequencediversityoftheHIvirusforTcellassays(e.g.ELISPOT).ProteinsequencesofallsubtypesofHIV-1/SIVcpzbasedontheHIVsequencedatabaseoftheLosAlamosNationalLabwereused.WedevelopedaproprietaryalgorithmtoobtainpeptidecombinationsprovidinganoptimalcoverageofproteinvariABIlity.
Amount:1vialcontaining15nmol(appr.25µg)perpeptide
Purity:>70%(HPLC-MS)
DeliveryFormat:Freezedriedinambertypeglassvial
Application(s):T-cellassays,Immunemonitoring,AntigenspecificT-cellstimulation,T-cellexpansion,Cellularimmuneresponse
Indication(s)/Topic(s):Infection,AIDS,Control
DeliveryTime:10-12days

PepMixes™HIVUltra
Thehumanimmunodeficiencyvirus(HIV)isthecausativeagentoftheacquiredimmunodeficiencysyndrome(AIDS),adisease,bywhichover25millionpeoplewerekilledinthepastthreedecades.ThemajordifficultywithHIVistheextremelyhighmutationrateoftheviralgenomeleADIngtoanenormousdiversityoftheHIVproteome,andallowingthevirustoescapethehumanimmuneresponse.Conventionalconsensusbasedpeptidepoolsaddressthisproblemonlyverypoorly.Therefore,whendesigningthenewHIV-1ENV,GAG,NEFandPOLUltraPepMix™PeptidePools,wefocusedprimarilyoncoverageofthetremendoussequencediversityofHIV.ProteinsequencesofallsubtypesofHIV-1/SIVcpzfromtheLosAlamosNationalLabwereconsidered.Wedevelopedaproprietaryalgorithmtoobtainpeptidecombinationsprovidingoptimalcoverageoftheproteinvariability.BroadcladecoverageprovidedbythesenewpeptidepoolsenablesstimulationandmonitoringofimmuneresponsesinHIVpatientsindependentofgeographicalorigin.

BenefitsofPepMix™
-EquivalentorbetterstimulationofCD4+andCD8+T-cellresponsescomparedtowholeproteinantigens
-SimultaneousdetectionofCD4+andCD8+responsesinasinglesample
-ImprovedresponsesinstoredbloodandPBMcellscomparedtowholeproteinantigens
-Morereliableascontrolthanphytohemagglutinin(PHA)andconcanavalinA(ConA)
-High-batch-to-batchreproducibility
-Reliablechemicalandbiochemicalqualitycontrolandqualityassuranceforpeptidesynthesisandpeptidepoolgeneration
-Prolongedshelfstabilitywhenstoredfreezedried

PepMix™HIV(NEF)Ultra

SelectedReferences
"InVitroStudiesoftheImpactofMaribavironCMV-SpecificCellularImmuneResponses"
Stacheletal.,JClinVirol. (2015)-PMID:26780109
"InductionOfBroad-basedImmunityandProtectiveEfficacybySelf-amplifyingmRNAVaccinesEncodingInfluenzaVirusHemagglutinin"
Brazzolietal.,JournalofVirology (2015)-PMID:26468547
"TargetingofNucleoproteintoChemokineReceptorsbyDNAVaccinationResultsinIncreasedCD8+-mediatedCrossProtectionAgainstInfluenza"
Baranowskaetal.,Vaccine(2015)-PMID:26387432
"CharacteristicsofImmuneMemory10-15yearsAfterPrimaryHepatitisBVaccination"
Hummeletal.,Vaccine(2015)-PMID:n.a.
"TCellsSpecificforDifferentLatentandLyticViralProteinsEfficientlyControlEpstein-BarrVirusTransformedBCells"
Nowakowskaetal.,Cytotherapy(2015)-PMID:26276009
"High-densityPrecultureofPBMCsRestoresDefectiveSensitivityofCirculatingCD8TCellstoVirus-andTumor-derivedAntigens"
Wegneretal.,Blood(2015)-PMID:26024876
"MetabolicRegulationofHepatitisBImmunopathologybyMyeloid-derivedSuppressorCells"
Pallettetal.,NatureMedicine(2015)-PMID:25962123

ApplicationNotes
"DevelopingMulti-HIVAntigenSpecificT-CellsasaComponentofaCureStrategy"
Lametal.(2015)Fulltext
"Peptide-stimulatedExpansionofVirus-specificTcellsForPreventativeTreatmentAfterAllogeneicStemCellTransplantation"
Garyetal.(2015)Fulltext

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Testimonials
"Toestablishanovelroleformyeloidderivedsuppressorcells(Pallettetal,Nat.Med.2015)inchronicviralinfection,weutilisedthePepMixCEFPool(extended)aswellasacustomsynthesizedPepMixspanningthecoreregionofHBVgenotypeD.Whereas,theCEFpeptidepoolconsistsof32peptides,eachcorrespondingtoadefinedHLAclassI-restrictedT-cellepitopefromCytomegalo,Epstein-Barr,andInfluenzavirus,thelattercustomPepMixincluded15mericpeptidesoverlappingby10aminoacids.SpecificallythiscompositionenabledustomonitorboththeantiviralCD8+andCD4+TcellresponsesinchronicHBVinfectionandthenon-antigenspecificTcellsthatareknowntomediateimmunopathologyintheliver.OurentireexperiencewithJPT,fromordering/deliverytouseinthelabwasexcellent.Notonlywerethereagentsabletoperformreliablyandconsistentlyinvitrofrombatchtobatch,thecustomerandtechnicalsupportprovidedwascontinuallyavailableandefficientwhenneeded.JPTwillremainour"go-to"companyforpurchasingpeptides."
Dr.LauraJPallett,InfectionandImmunity,UniversityCollegeLondon,UK

PepMix™HIV(NEF)Ultra

TechnicalData:
ApplicationAntigenspecificT-cellstimulation
Immunemonitoring
T-cellassays
T-cellexpansion
ProteinNameNefprotein(NEF)
OrganismHumanimmunodeficiencyvirus(HIV)
Indication/TopicAIDS(HIV)
Numberofpeptides150 

FurtherInformation:
Amount1vialcontaining15nmol(appr.25µg)perpeptide
SpecificationsSelectedimmunogenicpeptides(15mers)
DocumentationProtocol,DatasheetandAnnotations
SequenceSeeDatasheetunderProduct
Documentation
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