ProductCode:PM-CEA
Poolof173peptidesderivedfromapeptidescan(15merswith11aaoverlap)throughCarcinoembryonicantigen-relatedcelladhesionmolecule5(Swiss-ProtID:P06731)ofHomosapiens(Human).
Amount:1vialcontaining15nmol(appr.25µg)perpeptide
Purity:>70%(HPLC-MS)
DeliveryFormat:Freezedriedinambertypeglassvial
Application(s):T-cellassays,Immunemonitoring,AntigenspecificT-cellstimulation,T-cellexpansion,Cellularimmuneresponse
Indication(s)/Topic(s):Cancer,Epithelium
DeliveryTime:2-5days
PepMixes™PeptidePools
Theuseofcompleteprotein-spanningmixturesofoverlappingpeptides(PepMix™)isextremelyefficientforimmunostimulationofT-lymphocytesanddiagnosticapplications.InordertoestablishreliableandvalidatedT-cellassayssuchasELISPOT,appropriatepositiveandnegativecontrolsareessential.SelectionofsuchcontrolshelpstoconfirmproperfunctionalityoftheassayaswellasviABIlityofthecellsused.Inaddition,highqualitycontrolsaremostimportanttoestablishstandardizedassayprotocolsinclinicalsetups.Comparedtocommonlyusedcontrolslikephytohemagglutinin(PHA)andconcanavalinA(ConA)thatstimulateT-cellsaswellasothercelltypesorfulllengthantigens,syntheticpeptidepoolsoffertheadvantageofahighbatch-to-batchreproducibility,applicabilityofreliablechemicalandbiochemicalQC/QAmeasuresandshelfstabilitywhenstoredasfreezedriedaliquots.
BenefitsofPepMix™
-EquivalentorbetterstimulationofCD4+andCD8+T-cellresponsescomparedtowholeproteinantigens
-SimultaneousdetectionofCD4+andCD8+responsesinasinglesample
-ImprovedresponsesinstoredbloodandPBMcellscomparedtowholeproteinantigens
-Morereliableascontrolthanphytohemagglutinin(PHA)andconcanavalinA(ConA)
-High-batch-to-batchreproducibility
-Reliablechemicalandbiochemicalqualitycontrolandqualityassuranceforpeptidesynthesisandpeptidepoolgeneration
-Prolongedshelfstabilitywhenstoredfreezedried
SelectedReferences
"DysfunctionofPSA-specificCD8+TCellsinProstateCancerPatientsCorrelateswithCD38andTim-3Expression"
Jappetal.,CancerImmunolImmunother.(2015)-PMID:26289091
"AnHPV-E6/E7ImmunotherapyPlusPD-1CheckpointInhibitionResultsinTumorRegressionandReductioninPD-L1Expression"
Riceetal.,CancerGeneTherapy(2015)-PMID:26337747
"Survivin-specificCD4+TCellsareDecreasedinPatientswithSurvivin-positiveMyeloma"
Lockeetal.,JImmunotherCancer(2015)-PMID:25992291
"APhaseITrialCombiningDecitabine/dendriticCellVaccineTargetingMAGE-A1,MAGE-A3andNY-ESO-1forChildrenwithRelapsedorTherapy-RefractoryNeuroblastomaandSarcoma"
Krishnadasetal.,CancerImmunolImmunother.(2015)-PMID:26105625
"GuidelinesFortheAutomatedEvaluationofElispotAssays"
Janetzkietal.,NatureProtocols(2015)-PMID:26110715
"PresenceofCirculatingHer-2-reactiveCD8+T-CellsisAssociatedwithLowerFrequenciesofMyeloid-derivedSuppressorCellsandRegulatoryTCells,andBetterSurvivalinElderlyBreastCancerPatients"
KiniBailuretal.,BreastCancerResearch(2015)-PMID:n.a.
"STAT3SignalingContributestotheHighEffectorActivitiesofInterleukin-15-derivedDendriticCells"
Okadaetal.,ImmunolCellBiol.(2015)-PMID:25582338
ApplicationNotes
"DevelopingMulti-HIVAntigenSpecificT-CellsasaComponentofaCureStrategy"
Lametal.(2015)Fulltext
"Peptide-stimulatedExpansionofVirus-specificTcellsForPreventativeTreatmentAfterAllogeneicStemCellTransplantation"
Garyetal.(2015)Fulltext
Testimonials
"Toestablishanovelroleformyeloidderivedsuppressorcells(Pallettetal,Nat.Med.2015)inchronicviralinfection,weutilisedthePepMixCEFPool(extended)aswellasacustomsynthesizedPepMixspanningthecoreregionofHBVgenotypeD.Whereas,theCEFpeptidepoolconsistsof32peptides,eachcorrespondingtoadefinedHLAclassI-restrictedT-cellepitopefromCytomegalo,Epstein-Barr,andInfluenzavirus,thelattercustomPepMixincluded15mericpeptidesoverlappingby10aminoacids.SpecificallythiscompositionenabledustomonitorboththeantiviralCD8+andCD4+TcellresponsesinchronicHBVinfectionandthenon-antigenspecificTcellsthatareknowntomediateimmunopathologyintheliver.OurentireexperiencewithJPT,fromordering/deliverytouseinthelabwasexcellent.Notonlywerethereagentsabletoperformreliablyandconsistentlyinvitrofrombatchtobatch,thecustomerandtechnicalsupportprovidedwascontinuallyavailableandefficientwhenneeded.JPTwillremainour"go-to"companyforpurchasingpeptides."
Dr.LauraJPallett,InfectionandImmunity,UniversityCollegeLondon,UK
Application | AntigenspecificT-cellstimulation CancerbioMarkerresearch Immunemonitoring T-cellassays T-cellexpansion |
ProteinName | Carcinoembryonicantigen-relatedcelladhesionmolecule5 |
Organism | Homosapiens(Human) |
Indication/Topic | Cancer Epithelium |
Numberofpeptides | 173 |
Amount | 1vialcontaining15nmol(appr.25µg)perpeptide |
Specifications | Peptidescan(15merswith11aaoverlap) |
Documentation | ProtocolandDatasheet |
Swiss-ProtID | P06731 |
NCBI | AAH34671 |
Sequence | MESPSAPPHRWCIPWQRLLLTASLLTFW NPPTTAKLTIESTPFNVAEGKEVLLLVH NLPQHLFGYSWYKGERVDGNRQIIGYVI GTQQATPGPAYSGREIIYPNASLLIQNI IQNDTGFYTLHVIKSDLVNEEATGQFRV YPELPKPSISSNNSKPVEDKDAVAFTCE PETQDATYLWWVNNQSLPVSPRLQLSNG NRTLTLFNVTRNDTASYKCETQNPVSAR RSDSVILNVLYGPDAPTISPLNTSYRSG ENLNLSCHAASNPPAQYSWFVNGTFQQS TQELFIPNITVNNSGSYTCQAHNSDTGL NRTTVTTITVYAEPPKPFITSNNSNPVE DEDAVALTCEPEIQNTTYLWWVNNQSLP VSPRLQLSNDNRTLTLLSVTRNDVGPYE CGIQNELSVDHSDPVILNVLYGPDDPTI SPSYTYYRPGVNLSLSCHAASNPPAQYS WLIDGNIQQHTQELFISNITEKNSGLYT CQANNSASGHSRTTVKTITVSAELPKPS ISSNNSKPVEDKDAVAFTCEPEAQNTTY LWWVNGQSLPVSPRLQLSNGNRTLTLFN VTRNDARAYVCGIQNSVSANRSDPVTLD VLYGPDTPIISPPDSSYLSGANLNLSCH SASNPSPQYSWRINGIPQQHTQVLFIAK ITPNNNGTYACFVSNLATGRNNSIVKSI TVSASGTSPGLSAGATVGIMIGVLVGVA LI |
ProteinLength(aa) | 702 |