JPT/RepliTope™ EBV (LMP1)/RT-EBV-LMP1/
RepliTope™EBV(LMP1)
ProductCode:RT-EBV-LMP1
Peptidemicroarraydisplaying94peptidesderivedfromapeptidescan(15merswith11aaoverlap)throughLatentmembraneprotein1(LMP1)(Swiss-ProtID:P03230)ofEpstein-Barrvirus(HHV4).
Amount:1peptidemicroarray(eachpeptideisdeposited3xpersubarray,4subarraysperslide)+dummyslideandspacers(neededforincubation)
Purity:Freeoftruncatedsequences
DeliveryFormat:Peptidesimmobilizedontoglassslide(3x1inches;75by25mm)
Application(s):Immunemonitoring,Humoralimmuneresponse,SEROMarkerdiscovery,Antibodyepitopediscovery,Bindingexperiments
Indication(s)/Topic(s):Infection,Cancer,Hodgkin"slymphoma,Burkitt"slymphoma,Nasopharyngealcarcinoma,Dermatomyositis,Systemiclupuserythematosus,Rheumatoidarthritis,Sjgren"ssyndrome,Multiplesclerosis
DeliveryTime:10-12days
RepliTope™PeptideMicroarrays
JPTPeptideTechnologiesappliesitsuniquepeptidemicroarrayplatformtogeneratepeptidemicroarraysonglassslidesforbiomarkerdiscovery,immunomonitoringanddetectionandvalidationofproteininteractions.PeptidesareimmobilizedonglassslidesviaaflexIBLelinkerbychemoselectivecoupling.Thecovalentlyattachedpeptidesaredisplayedinfourcopiesoneachslide.Inaddition,multipleidenticalcopiesofeachmicroarrayareproduced.Incubationcanbeperformedmanuallyorautomatedusingonlyminimalamountsofyoursample.Read-outviafluorescenceresultsinlowbackgroundandhighsensitivity.
BenefitsofJPT"sRepliTope™PeptideMicroarrays
-Gethundredsofidenticalmicroarraycopies
-Peptidesarefreeoftruncatedsequences
-Smallamountsofyourprecioussamplesareneededforincubation
-ApplicabletoavarietyofBIOLOGicalsamples(e.g.serum,plasma,antibody)
-Lowbackgroundonglasssurface
-Read-outviafluorescence
RepliTope™EBV(LMP1)
SelectedReferencesforJPT"sPeptideMicroarrays
"StructureofGPN-loopGTPaseNpa3andImplicationsforRNAPolymeraseIIAssembly"
Niessertal.,MolCellBiol. (2015)-PMID:26711263
"ProtectiveEfficacyofAdenovirus-proteinVaccinesAgainstSIVChallengesinRhesusMonkeys"
Barouchetal.,Science(2015)-PMID:26138104
"IdentificationandCharacterizationofCommonBCellEpitopeinBovineLeukemiaVirusviaHigh-throughputPeptideScreeningSysteminInfectedCattle"
Baietal.,Retrovirology(2015)-PMID:26715158
"High-ThroughputMicroarrayIncubationsUsingMulti-WellChambers"
Zerwecketal.,MethodsMolBiol.(2015)-PMID:26490464
"PeptideMicroarraysforCoverageofSequenceDiversityinMonitoringtheBcellImmuneResponse(TECH2P.911)"
Castro,etal.,TheJournalofImmunology(2015)-PMID:n.a.
"AntibodiestoInfluenzaNucleoproteinCross-reactwithHumanHypocretinReceptor2"
Ahmedetal.,ScienceTranslationalMedicine(2015)-PMID:26136476
"SerumReactomeInducedbyBordetellaPertussisInfectionandPertussisVaccines:QualitativeDifferencesinSerumAntibodyRecognitionPatternsRevealedbyPeptideMicroarrayAnalysis"
Valentinietal.,BMCImmunol.(2015)-PMID:26129684
ApplicationNotes
"AModularApproachforEpitopeDiscoveryandHigh-ResolutionProfilingofHumoralImmuneResponses"
Pawlowskietal.(2013)Fulltext
"MultipleSclerosisandEpsteinBarrVirusInfection.AnEpitopeMappingStudy"
Reimeretal.(2014)Fulltext
"TheChallengeofAntigenSequenceDiversity:SolutionswithULTRAPeptideLibraries"
Pawlowskietal.(2015)Fulltext
"RapidMimotopeOptimizationforPharmacokineticAnalysisoftheNovelTherapeuticantibodyIMAB362"
Danescharetal.(2014)Fulltext
TestimonialsforJPT"sPeptideMicroarrays
"Overthepastfiveyearsmyresearchteamhasbeenactiveinthedevelopmentofspecies-specificpeptidearrays.OurABIlitytoadvancethistechnologyintonewresearchareashasbeendependentonworkingwiththehighestqualityofarrays.Inthisregard,JPT"scommitmenttoexcellencehasbeenacriticalfoundationforthesuccessofourresearchprogram.Ihavebeenconsistentlyimpressedwiththeircustomerservice,technicalexpertiseandprofessionalism.Whenitcomestopeptideworktherearenootheroptionsforourgroup,JPTisthebest."
ScottNapper,PhD,VaccineandInfectiousDiseaseOrganization(VIDO),UniversityofSaskatchewan,Canada
"TheRV144HIVtrialisconsideredasoneoffirstsuccessfulHIVvaccinetrials.IthasbecomeclearthattheV2loopofgp120isanimportantsiteforimmunogenicityandprotectionfromHIVinfection.TheuseofJPT"sPepStar™microarraytechnologyhasbeenveryusefulforthecorrelationoftheclincialoutcomewithhumoralimmuneresponses.AshavethecyclicpeptidesbeenfromJPTtovalidatethesefindings!."
JeffCurrier,PhD,WalterReedArmyInstitute,Rockville,Maryland,USA
"WestudymodulationofdopaminergicneurotransmissonbyTAAR1andD2R.ThefunctionalinteractionofthetworeceptorsinthebrainsupportsTAAR1asatargetforthetreatmentofpsychiatricdisorderssuchasschizophreniaorbipolardisorder.Tomaptheepitopesofournewlygeneratedspecificanti-ratTAAR1antibodiesweusedJPT"sPepStarTMpeptidemicroarrays.Thepeptidemicroarraysgreatlycontributedtooursuccessfulandrecentlypublishedstudy.WewereverysatisifiedwiththeexceptionalproductandservicedeliveredbyJPTPeptideTechnologiesaswellastheirscientificCustomerSupportwhichwasalwaysatourdisposal."
StefanObermüller,F.Hoffmann-LaRocheLtd.,RochePharmaResearchandEarlyDevelopment,Basel,Switzerland
RepliTope™EBV(LMP1)
| Application | Antibodyepitopediscovery Immunemonitoring |
| ProteinName | Latentmembraneprotein1(LMP1) |
| Organism | Epstein-Barrvirus(HHV-4) |
| Indication/Topic | Autoimmunedisease Burkitt"slymphoma Hodgkin"slymphoma Nasopharyngealcarcinoma |
| Numberofpeptides | 94 |
FurtherInformation:
| Amount | 1peptidemicroarray(eachpeptideisdeposited3xpersubarray,4subarraysperslide)+dummyslideandspacers(neededformanualincubation) |
| Specifications | Peptidescan(15merswith11aaoverlap) |
| Documentation | CDwithProtocol,Datasheet,gal-fileandgal-viewer |
| Swiss-ProtID | P03230 |
| NCBI | YP_401722 |
| Sequence | MEHDLERGPPGPRRPPRGPPLSSSLGLA LLLLLLALLFWLYIVMSDWTGGALLVLY SFALMLIIIILIIFIFRRDLLCPLGALC ILLLMITLLLIALWNLHGQALFLGIVLF IFGCLLVLGIWIYLLEMLWRLGATIWQL LAFFLAFFLDLILLIIALYLQQNWWTLL VDLLWLLLFLAILIWMYYHGQRHSDEHH HDDSLPHPQQATDDSGHESDSNSNEGRH HLLVSGAGDGPPLCSQNLGAPGGGPDNG PQDPDNTDDNGPQDPDNTDDNGPHDPLP QDPDNTDDNGPQDPDNTDDNGPHDPLPH SPSDSAGNDGGPPQLTEEVENKGGDQGP PLMTDGGGGHSHDSGHGGGDPHLPTLLL GSSGSGGDDDDPHGPVQLSYYD |
| ProteinLength(aa) | 386 |
Acccorporation/2,6-DIFLUORO-3-METHOXYBENZENEBORONIC ACID/1G/BOR0000941-1G
TriLink/2-Amino-2-deoxycytidine-5-Triphosphate - (N-1026)/10 µmole/N-1026-10
TriLink/Pseudouridine-5-Triphosphate - (N-1019)/5 µmole/N-1019-5
TriLink/2-Fluoro-2-deoxyuridine-5-Triphosphate - (N-1010)/5 x 10 µmole/N-1010-50
4adi/Anti-Human alpha-Tubulin IgG-Biotin Conjugate/100 ul/TUBA13-BTN
GiottoBiotech/MBP - Maltose Binding Protein/G03MPB01cn_t5