ProductCode:RT-EBV-EBNA3b
Peptidemicroarraydisplaying234peptidesderivedfromapeptidescan(15merswith11aaoverlap)throughEpstein-Barrnuclearantigen4(EBNA3b)(Swiss-ProtID:Q1HVG4)ofEpstein-Barrvirus(HHV4).
Amount:1peptidemicroarray(eachpeptideisdeposited3xpersubarray,4subarraysperslide)+dummyslideandspacers(neededforincubation)
Purity:Freeoftruncatedsequences
DeliveryFormat:Peptidesimmobilizedontoglassslide(3x1inches;75by25mm)
Application(s):Immunemonitoring,Humoralimmuneresponse,SeroMarkerdiscovery,Antibodyepitopediscovery,Bindingexperiments
Indication(s)/Topic(s):Infection,Cancer,Hodgkinslymphoma,Burkittslymphoma,Nasopharyngealcarcinoma,Dermatomyositis,Systemiclupuserythematosus,Rheumatoidarthritis,Sjgrenssyndrome,Multiplesclerosis
DeliveryTime:10-12days
RepliTope™PeptideMicroarrays
JPTPeptideTechnologiesappliesitsuniquepeptidemicroarrayplatformtogeneratepeptidemicroarraysonglassslidesforbiomarkerdiscovery,immunomonitoringanddetectionandvalidationofproteininteractions.Peptidesareimmobilizedonglassslidesviaaflexiblelinkerbychemoselectivecoupling.Thecovalentlyattachedpeptidesaredisplayedinfourcopiesoneachslide.Inaddition,multipleidenticalcopiesofeachmicroarrayareproduced.Incubationcanbeperformedmanuallyorautomatedusingonlyminimalamountsofyoursample.Read-outviafluorescenceresultsinlowbackgroundandhighsensitivity.
BenefitsofJPTsRepliTope™PeptideMicroarrays
-Gethundredsofidenticalmicroarraycopies
-Peptidesarefreeoftruncatedsequences
-Smallamountsofyourprecioussamplesareneededforincubation
-Applicabletoavarietyofbiologicalsamples(e.g.serum,plasma,antibody)
-Lowbackgroundonglasssurface
-Read-outviafluorescence
SelectedReferencesforJPTsPeptideMicroarrays
"StructureofGPN-loopGTPaseNpa3andImplicationsforRNAPolymeraseIIAssembly"
Niessertal.,MolCellBiol. (2015)-PMID:26711263
"ProtectiveEfficacyofAdenovirus-proteinVaccinesAgainstSIVChallengesinRhesusMonkeys"
Barouchetal.,Science(2015)-PMID:26138104
"IdentificationandCharacterizationofCommonBCellEpitopeinBovineLeukemiaVirusviaHigh-throughputPeptideScreeningSysteminInfectedCattle"
Baietal.,Retrovirology(2015)-PMID:26715158
"High-ThroughputMicroarrayIncubationsUsingMulti-WellChambers"
Zerwecketal.,MethodsMolBiol.(2015)-PMID:26490464
"PeptideMicroarraysforCoverageofSequenceDiversityinMonitoringtheBcellImmuneResponse(TECH2P.911)"
Castro,etal.,TheJournalofImmunology(2015)-PMID:n.a.
"AntibodiestoInfluenzaNucleoproteinCross-reactwithHumanHypocretinReceptor2"
Ahmedetal.,ScienceTranslationalMedicine(2015)-PMID:26136476
"SerumReactomeInducedbyBordetellaPertussisInfectionandPertussisVaccines:QualitativeDifferencesinSerumAntibodyRecognitionPatternsRevealedbyPeptideMicroarrayAnalysis"
Valentinietal.,BMCImmunol.(2015)-PMID:26129684
ApplicationNotes
"AModularApproachforEpitopeDiscoveryandHigh-ResolutionProfilingofHumoralImmuneResponses"
Pawlowskietal.(2013)Fulltext
"MultipleSclerosisandEpsteinBarrVirusInfection.AnEpitopeMappingStudy"
Reimeretal.(2014)Fulltext
"TheChallengeofAntigenSequenceDiversity:SolutionswithULTRAPeptideLibraries"
Pawlowskietal.(2015)Fulltext
"RapidMimotopeOptimizationforPharmacokineticAnalysisoftheNovelTherapeuticantibodyIMAB362"
Danescharetal.(2014)Fulltext
TestimonialsforJPTsPeptideMicroarrays
"Overthepastfiveyearsmyresearchteamhasbeenactiveinthedevelopmentofspecies-specificpeptidearrays.Ourabilitytoadvancethistechnologyintonewresearchareashasbeendependentonworkingwiththehighestqualityofarrays.Inthisregard,JPTscommitmenttoexcellencehasbeenacriticalfoundationforthesuccessofourresearchprogram.Ihavebeenconsistentlyimpressedwiththeircustomerservice,technicalexpertiseandprofessionalism.Whenitcomestopeptideworktherearenootheroptionsforourgroup,JPTisthebest."
ScottNapper,PhD,VaccineandInfectiousDiseaseOrganization(VIDO),UniversityofSaskatchewan,Canada
"TheRV144HIVtrialisconsideredasoneoffirstsuccessfulHIVvaccinetrials.IthasbecomeclearthattheV2loopofgp120isanimportantsiteforimmunogenicityandprotectionfromHIVinfection.TheuseofJPTsPepStar™microarraytechnologyhasbeenveryusefulforthecorrelationoftheclincialoutcomewithhumoralimmuneresponses.AshavethecyclicpeptidesbeenfromJPTtovalidatethesefindings!."
JeffCurrier,PhD,WalterReedArmyInstitute,Rockville,Maryland,USA
"WestudymodulationofdopaminergicneurotransmissonbyTAAR1andD2R.ThefunctionalinteractionofthetworeceptorsinthebrainsupportsTAAR1asatargetforthetreatmentofpsychiatricdisorderssuchasschizophreniaorbipolardisorder.Tomaptheepitopesofournewlygeneratedspecificanti-ratTAAR1antibodiesweusedJPTsPepStarTMpeptidemicroarrays.Thepeptidemicroarraysgreatlycontributedtooursuccessfulandrecentlypublishedstudy.WewereverysatisifiedwiththeexceptionalproductandservicedeliveredbyJPTPeptideTechnologiesaswellastheirscientificCustomerSupportwhichwasalwaysatourdisposal."
StefanObermüller,F.Hoffmann-LaRocheLtd.,RochePharmaResearchandEarlyDevelopment,Basel,Switzerland
Application | Antibodyepitopediscovery Immunemonitoring |
ProteinName | Epstein-Barrnuclearantigen(EBNA) |
Organism | Epstein-Barrvirus(HHV-4) |
Indication/Topic | Autoimmunedisease Burkittslymphoma Hodgkinslymphoma Nasopharyngealcarcinoma |
Numberofpeptides | 234 |
Amount | 1peptidemicroarray(eachpeptideisdeposited3xpersubarray,4subarraysperslide)+dummyslideandspacers(neededformanualincubation) |
Specifications | Peptidescan(15merswith11aaoverlap) |
Documentation | CDwithProtocol,Datasheet,gal-fileandgal-viewer |
Swiss-ProtID | Q1HVG4 |
NCBI | ABB89244 |
Sequence | MKKAWLSRAQQADAGGASGSEDPPDYGD QGNVQQVGSDPISPAIGPFELSAASEDD PQSGPVEENLDAAAREEEEPDEQEHNGG DDPLEVHTRQPRFVDVNPTQAPVIQLVH AVYDSMLQSDLRSLGSLFLEQNLNIEEF IWMCMTVRHRCQAIRQKPLPIDKQRRWK LLSPYRTWRMGYRTQTLNVNSFETGGDK VHPLLVTATLGCEEGLRHAITYSAGIVQ LPRMSDQNQKIETAFLMARRARSLSAER YTLFFDLVSSGNTLYAIWIGLGTRNRVA FVEFVGWLCKKDHTHIREWFRQCTGRPS PSKPWMRAHPVAVPYDDPLTSEETDLAY ARGLAMSIEAARLPDDPIIVEDDDESEE IEDKCDKDEEESGTEDVTSIPQTLPHSP TVYGRPSVFYRKPDTKSTKKCRAIVTDL SIIKVIEDEHRKKKTARTEQPRAKPDSP APTVVLRRPPTQKVTGPAGSLSVQAQLE PWQPLSWPHETRVILHGPPTQGDQAHGS MLDLLEKDDQHMEQQVMATLLPPEPHQP RSGRRAPCVYTADLDIESDEPATSEPVL DQLLPAPGLGPLAIQPLTSPTTSQLRSS APSHAQTPWPVTHPSQTPGGPTTQSLAP ETEAPRQWPMPLRPIPLHPLRMQPISFN PAVRPTPHQPPQVEPTFYQSTWVKPPQQ YQPQMGHIPYQPRPTGHSTMLRPQWAPT TMQPPPRAPTPMPPPQGPPTAMQRPQGA PTPMPPPQGPPTAMQRPRGAPTPMPPPQ GPPTAMQLSPRALTGQKGPAKHILRQLL TGGVKSGRPSLKFKAALERQAAAGLRPS PGSGTGAKIVQAPVFYPPVLQPIQVMWQ VGSSKAVAASTVTQAPTEYTGERRLGGP MSPTDIPPSKRVKKKAYPERKTPHGGPS HSSTVMWENVSQGQQQTLECGGTDKQER NMLGMGDIAVSSPSSSETSNDE |
ProteinLength(aa) | 946 |