JPT/RepliTope™ HIV1 env (gp120 protein + signal peptide)/RT0014/

RepliTope™HIV-1env(gp120protein+signalpeptide)

ProductCode:RT-0014

Peptidemicroarraydisplaying164peptidesderivedfromapeptidescan(15merswith11aaoverlap)throughgp120protein(Swiss-ProtID:P04578,region:1-500)ofHumanimmunodeficiencyvirus.
Amount:1peptidemicroarray(eachpeptideisdeposited3xpersubarray,4subarraysperslide)+dummyslideandspacers(neededforincubation)
Purity:Freeoftruncatedsequences
DeliveryFormat:Peptidesimmobilizedontoglassslide(3x1inches;75by25mm)
Application(s):Immunemonitoring,Humoralimmuneresponse,SeroMarkerdiscovery,Antibodyepitopediscovery,Bindingexperiments
Indication(s)/Topic(s):Infection,AIDS,Control
DeliveryTime:10-12days

RepliTope™PeptideMicroarrays
JPTPeptideTechnologiesappliesitsuniquepeptidemicroarrayplatformtogeneratepeptidemicroarraysonglassslidesforbiomarkerdiscovery,immunomonitoringanddetectionandvalidationofproteininteractions.Peptidesareimmobilizedonglassslidesviaaflexiblelinkerbychemoselectivecoupling.Thecovalentlyattachedpeptidesaredisplayedinfourcopiesoneachslide.Inaddition,multipleidenticalcopiesofeachmicroarrayareproduced.Incubationcanbeperformedmanuallyorautomatedusingonlyminimalamountsofyoursample.Read-outviafluorescenceresultsinlowbackgroundandhighsensitivity.

BenefitsofJPTsRepliTope™PeptideMicroarrays
-Gethundredsofidenticalmicroarraycopies
-Peptidesarefreeoftruncatedsequences
-Smallamountsofyourprecioussamplesareneededforincubation
-Applicabletoavarietyofbiologicalsamples(e.g.serum,plasma,antibody)
-Lowbackgroundonglasssurface
-Read-outviafluorescence

RepliTope™HIV-1env(gp120protein+signalpeptide)

SelectedReferencesforJPTsPeptideMicroarrays
"StructureofGPN-loopGTPaseNpa3andImplicationsforRNAPolymeraseIIAssembly"
Niessertal.,MolCellBiol. (2015)-PMID:26711263
"ProtectiveEfficacyofAdenovirus-proteinVaccinesAgainstSIVChallengesinRhesusMonkeys"
Barouchetal.,Science(2015)-PMID:26138104
"IdentificationandCharacterizationofCommonBCellEpitopeinBovineLeukemiaVirusviaHigh-throughputPeptideScreeningSysteminInfectedCattle"
Baietal.,Retrovirology(2015)-PMID:26715158
"High-ThroughputMicroarrayIncubationsUsingMulti-WellChambers"
Zerwecketal.,MethodsMolBiol.(2015)-PMID:26490464
"PeptideMicroarraysforCoverageofSequenceDiversityinMonitoringtheBcellImmuneResponse(TECH2P.911)"
Castro,etal.,TheJournalofImmunology(2015)-PMID:n.a.
"AntibodiestoInfluenzaNucleoproteinCross-reactwithHumanHypocretinReceptor2"
Ahmedetal.,ScienceTranslationalMedicine(2015)-PMID:26136476
"SerumReactomeInducedbyBordetellaPertussisInfectionandPertussisVaccines:QualitativeDifferencesinSerumAntibodyRecognitionPatternsRevealedbyPeptideMicroarrayAnalysis"
Valentinietal.,BMCImmunol.(2015)-PMID:26129684

ApplicationNotes
"AModularApproachforEpitopeDiscoveryandHigh-ResolutionProfilingofHumoralImmuneResponses"
Pawlowskietal.(2013)Fulltext
"MultipleSclerosisandEpsteinBarrVirusInfection.AnEpitopeMappingStudy"
Reimeretal.(2014)Fulltext
"TheChallengeofAntigenSequenceDiversity:SolutionswithULTRAPeptideLibraries"
Pawlowskietal.(2015)Fulltext
"RapidMimotopeOptimizationforPharmacokineticAnalysisoftheNovelTherapeuticantibodyIMAB362"
Danescharetal.(2014)Fulltext

TestimonialsforJPTsPeptideMicroarrays
"Overthepastfiveyearsmyresearchteamhasbeenactiveinthedevelopmentofspecies-specificpeptidearrays.Ourabilitytoadvancethistechnologyintonewresearchareashasbeendependentonworkingwiththehighestqualityofarrays.Inthisregard,JPTscommitmenttoexcellencehasbeenacriticalfoundationforthesuccessofourresearchprogram.Ihavebeenconsistentlyimpressedwiththeircustomerservice,technicalexpertiseandprofessionalism.Whenitcomestopeptideworktherearenootheroptionsforourgroup,JPTisthebest."
ScottNapper,PhD,VaccineandInfectiousDiseaseOrganization(VIDO),UniversityofSaskatchewan,Canada

"TheRV144HIVtrialisconsideredasoneoffirstsuccessfulHIVvaccinetrials.IthasbecomeclearthattheV2loopofgp120isanimportantsiteforimmunogenicityandprotectionfromHIVinfection.TheuseofJPTsPepStar™microarraytechnologyhasbeenveryusefulforthecorrelationoftheclincialoutcomewithhumoralimmuneresponses.AshavethecyclicpeptidesbeenfromJPTtovalidatethesefindings!."
JeffCurrier,PhD,WalterReedArmyInstitute,Rockville,Maryland,USA

"WestudymodulationofdopaminergicneurotransmissonbyTAAR1andD2R.ThefunctionalinteractionofthetworeceptorsinthebrainsupportsTAAR1asatargetforthetreatmentofpsychiatricdisorderssuchasschizophreniaorbipolardisorder.Tomaptheepitopesofournewlygeneratedspecificanti-ratTAAR1antibodiesweusedJPTsPepStarTMpeptidemicroarrays.Thepeptidemicroarraysgreatlycontributedtooursuccessfulandrecentlypublishedstudy.WewereverysatisifiedwiththeexceptionalproductandservicedeliveredbyJPTPeptideTechnologiesaswellastheirscientificCustomerSupportwhichwasalwaysatourdisposal."
StefanObermüller,F.Hoffmann-LaRocheLtd.,RochePharmaResearchandEarlyDevelopment,Basel,Switzerland