JPT/Histone Code Peptide Microarrays/His_MA_01/

HistoneCodePeptideMicroarrays

ProductCode:His_MA_01

Extensivepeptidelibrarycoveringallknownnaturalvariantsofhistonesequencesandmodificationsdisplayedononemicroarray(>3800peptides).
AminoAcidSequence:SeeProductDocumentation
Amount:1peptidemicroarray(eachpeptideisdeposited1xpersubarray,3subarraysperslide)+dummyslidesandspacers(neededformanualincubation)
DeliveryFormat:Peptidesimmobilizedontoglassslide(3x1inches;75by25mm)
Application(s):Bindingexperiments,Substrateidentification,Antibodyepitopediscovery,SubstrateSpecifity
Indication(s)/Topic(s):Cancer,HistoneDeacetylase,Histones,Generegulationandexpression,Epigenetics,Cellsignaling,Nucleosome
DeliveryTime:10-12days

HistoneCodePeptideMicroarray
Ashistonesareamajorfocusofepigenetics,JPTscomputationalscientistscompiledthisuniquelibraryofsyntheticpost-translationallymodifiedandunmodifiedhumanhistonepeptidesthatisnowavailableonasinglepeptidemicroarrayorasindividualbiotinylatedpeptides.Thepeptidelibraryconsistsofmorethan140020merpeptidesfromHistoneH1,H2A,H2B,H3andH4.PeptidescarryPTMsatreportedsites(e.g.acetylation,methylation,phosphorylation,citrullination...)invariouscombinationsrepresentingallpotentialvariants.Thislibraryissupplementedby2444peptidesfromanoverlappingpeptidescanthroughhistoneproteinscarryingnoorsinglemodificationsatreportedsites.

BenefitsofJPTsHistoneCodePeptideMicroarrays
-Gethundredsofidenticalmicroarraycopies
-Smallamountsofyourprecioussamplesareneededforincubation
-Applicabletoavarietyofbiologicalsamples(e.g.serum,lysate,purifiedproteins,antibodies)
-Lowbackgroundonglasssurface,read-outviafluorescence
-Unprecedentedresolutionformappingofprotein-histoneinteractionsduetocoverageofnaturalsequencevariants
-Screenthousandsofpotentialpeptidesubstrateseconomically

HistoneCodePeptideMicroarrays

SelectedReferences
"HistoneH2AandH4N-TerminalTailsarePositionedbytheMEP50WD-RepeatProteinforEfficientMethylationbythePRMT5ArginineMethyltransferase"
Burgosetal.,J.Biol.Chem.(2015)-PMID:25713080
"H3K23me2isaNewHeterochromaticMarkinCaenorhabditiselegans"
Vandammeetal., NucleicAcidsRes.(2015)-PMID:26476455
"StructureoftheArginineMethyltransferasePRMT5-MEP50RevealsaMechanismforSubstrateSpecificity"
Hoetal,PLOSONE(2013)-PMID:23451136
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistone-ModifyingEnzymeComplex"
Shechteretal.ApplicationNote(2013)(fulltext)

ApplicationNotes
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistone-ModifyingEnzymeComplex"
Shechteretal.ApplicationNote(2013)(fulltext)
"ComprehensiveCharacterizationofAntibodiesDirectedtowardsEpigeneticHistone-Modifications"
Maschetal.ApplicationNote(2015)(fulltext)

Testimonial
“JPTsnewextremelyhigh-densityhistonepeptidearrayisadramaticleapforwardforanalysisofthehistonecodehypothesis.Thecompletesequencecoverageacrosscorehistonesandhistonevariantsaswellasthepresenceofcommonandrarehistoneposttranslationalmodifications–aloneandincombination–makesthisanunparalleledtoolforstudyingthe“writers”and“readers”ofthehistonecode.MylaboratoryhasusedthesearraystoaskthesequenceandPTMdependenceofahistonewriterenzymeandwehavediscoveredanactivityspecifyingcodethatwillkeepusbusyforyearstocome.”
DavidShechter,PhD(DepartmentofBiochemistry,AlbertEinsteinCollegeofMedicine,YU,NY,USA)