JPT/Protease Substrate Set/PrSS36075/

ProteaseSubstrateSet

ProductCode:PrSS-360-75

Collectionof360internallyquenched(Dabcyl/EDANS)peptidesderivedfromproteolyticcleavagesites(fromP4toP4position)ofHomosapiens(Human).
Amount:1x384-wellMTP(75pmolperwell)
Purity:>95(HPLC-MS)
DeliveryFormat:384wellmicrotiterplate
Application(s):Cleavagesitedetection,Substrateidentification
Indication(s)/Topic(s):Protease
DeliveryTime:2-5days

EnzymeSubstrateSets
Oneofthemostefficientwaystostudyenzymeactivityandsubstratespecificityisincubatingtheenzymeofinterestwithmultiplesubstratepeptidesinparallel.JPTPeptideTechnologiesready-to-screenSubstrateSetsarecomprisedofnumeroussubstratepeptides,whichwerecarefullyselectedfromtheSwiss-ProtandPhosphobasedatabasesorbioinformaticallydesigned.TheSubstrateSetsaredeliveredinmicrotiterplatesfordirectincubationwithtargetenyzme.

BenefitsofJPTsEnzymeSubstrateSets
-Screennumerouspotentialenzymesubstratessimultaneouslyatlowcost
-SavetimeforHTS-Assaydevelopment
-Learnmoreaboutthebiologyofyourenzyme
-Read-outwithcommonELISAreaders
-Identificationofpotentialinvivosubstrates
-Determinekineticconstants

ProteaseSubstrateSet

SelectedReferences
"StructuralCharacterizationofZinc-boundZmp1,aZinc-dependentMetalloproteaseSecretedbyClostridiumDifficile"
Rubinoetal.,JBiolInorgChem.(2015)-PMID:26711661
"MatrixMetalloproteinase10DegradomicsinKeratinocytesandEpidermalTissueIdentifiesBioactiveSubstrateswithPleiotropicFunctions"
Schlageetal.,MolCellProteomics.(2015)-PMID:26475864

Testimonials
"Wewereverypleasedwithhoweasyitwastodotheexperiment(withKinaseSubstrateSets)usingtheequipmentwehadonhand-forexample,wejustusedour8-channelmicropipettetoaddtheenzyme/ATPmix."
BettyA.Eipper,Ph.D,UniversityofConnecticut,Farmington,USA

"Ourresearchreliesheavilyondevelopingrobusthigh-throughputscreenswithfluorescentpeptides. WehavefoundthatJPTsarethebestonthemarketbecausethesignal-to-noiseratioisveryhigh,providingthesensitivityweneedforthescreens.Theirpeptidesalwaysperformwell. Inaddition,theknowledge,wonderfulcustomersupport,andfastturnaroundtimeprovidedbyJPThavebeeninvaluableinhelpingusdevelopthebestpeptidesforourassays."
ProfessorCarlaKoehler,UCLA,LosAngeles,CA