JPT/Histone Peptide H2A 110:129 K119ac_pT120 (H2A.11BE1C1D2A3)/SPHis_0289/

HistonePeptideH2A110:129K119ac_pT120(H2A.11BE1C1D2A3)

ProductCode:SP-His_0289

C-terminallybiotinylatedsyntheticHistonepeptidecarrying2modifications(K119ac,pT120)derivedfromhumanHistoneH2A,H2A.11BE1C1D2A3(Swiss-ProtId(s):P0C0S8,Q7L7L0,P20671,Q6FI13,P04908,Q93077).
Aminoacidsequence:Ac-NIQAVLLPK-Lys(Ac)-pT-ESHHKAKGK-Lys(Biotinoyl)-NH2
Amount:1vialcontaining10nmol(appr.35µg)
Purity:>70%(HPLC-MS)
Deliveryformat:Freeze-driedinambertypeglassvials
Application(s):WesternBlot,Bindingexperiments,ELISA,Enzymekinetics,Pulldownassays,SubstrateSpecifity
Indication(s)/Topic(s):Cancer,Phosphatase,Kinase,Methyltransferase,Acetyltransferase,Histones,Phosphatase,Generegulation&expression,Epigenetics,Cellsignaling,Nucleosome,Aging
Deliverytime:2-5days

JPTsHistoneCodePeptides
Ashistonesareamajorfocusofepigenetics,JPTscomputationalscientistscompiledauniquelibraryofsyntheticposttranslationallymodifiedandunmodifiedhumanhistonepeptidesthatisnowavailableasindividualbiotinylatedpeptides.Thepeptidelibraryconsistsofmorethansevenhundred20merpeptidesfrom29histonefamilies(e.g.H2A[1,19,85...],H2B[1,22,42...],H3[1,23,43...],H4[1,14,44...]).PeptidescarryPTMsatreportedsites(e.g.acetylation,methylation,phosphorylation,citrullination)invariouscombinationsrepresentingnaturalvariants.JPTshighlyskilledandcommittedscientificstaffensuresthatthemostappropriatemethodsandtechniquesareselectedforeverysynthesisproject.AllofJPTscatalogpeptidesareprovidedwithHPLC-MSanalysestoconfirmtheidentityanddemonstratethehighqualityofourpeptides.

BenefitsofJPTsHistoneCodePeptides
-WholeproductionperformedinaregulatedEuropeanenvironmentaccordingtoDINENISO9001:2015&GCLPstandards;auditswelcome!
-Forhigh-throughputscreeningthewholeHistoneCodePeptidelibraryisalsoavailabledisplayedonpeptidemicroarrays
-Provisionoffreezedriedaliquotsforenhancedstability
-Proventrackrecordformanyapplications

HistonePeptideH2A110:129K119ac_pT120(H2A.11BE1C1D2A3)

SelectedReferencesforHistonePeptideTools
"HistoneH2AandH4N-TerminalTailsarePositionedbytheMEP50WD-RepeatProteinforEfficientMethylationbythePRMT5ArginineMethyltransferase"
Burgosetal.,J.Biol.Chem.(2015)-PMID:25713080
"H3K23me2isaNewHeterochromaticMarkinCaenorhabditiselegans"
Vandammeetal., NucleicAcidsRes.(2015)-PMID:26476455
"StructureoftheArginineMethyltransferasePRMT5-MEP50RevealsaMechanismforSubstrateSpecificity"
Hoetal.PLOSONE(2013)-PMID:23451136
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistone-ModifyingEnzymeComplex"
Shechteretal,ApplicationNote(2013)(fulltext)
"ComprehensivePeptideMicroarraysforHistoneResearch"
Schnatbaumetal,32thEPS(2012)(fulltext)

ApplicationNotes
"UseofHigh-DensityHistonePeptideArraysforParsingtheSpecificityofaHistone-ModifyingEnzymeComplex"
Shechteretal.ApplicationNote(2013)(fulltext)
"ComprehensiveCharacterizationofAntibodiesDirectedtowardsEpigeneticHistone-Modifications"
Maschetal.ApplicationNote(2015)(fulltext)

TestimonialforHistoneCodePeptideMicroarray
"JPTsnewextremelyhigh-densityhistonepeptidearrayisadramaticleapforwardforanalysisofthehistonecodehypothesis.Thecompletesequencecoverageacrosscorehistonesandhistonevariantsaswellasthepresenceofcommonandrarehistonepost-translationalmodifications "aloneandincombination"makesthisanunparalleledtoolforstudyingthe"writers"and"readers"ofthehistonecode.MylaboratoryhasusedthesearraystoaskthesequenceandPTMdependenceofahistonewriterenzymeandwehavediscoveredanactivityspecifyingcodethatwillkeepusbusyforyearstocome."
DavidShechter,PhD,DepartmentofBiochemistry,AlbertEinsteinCollegeofMedicine,YU(NY,USA)