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Product Code: PM-HIV-NEF
Pool of 150 15meric peptides derived from Nef protein designed to cover the high sequence diversity of the HI virus for T cell assays (e.g. ELISPOT). Protein sequences of all subtypes of HIV-1/SIVcpz based on the HIV sequence database of the Los Alamos National Lab were used. We developed a proprietary algorithm to obtain peptide combinations providing an optimal coverage of protein variability.
Amount: 1 vial containing 15 nmol (appr. 25µg) per peptide
Purity: >70% (HPLC-MS)
Delivery Format: Freeze dried in amber type glass vial
Application(s): T-cell assays, Immune monitoring, Antigen specific T-cell stimulation, T-cell expansion, Cellular immune response
Indication(s)/Topic(s): Infection, AIDS, Control
Delivery Time: 10 - 12 days
PepMixes™ HIV Ultra
The human immunodeficiency virus (HIV) is the causative agent of the acquired immunodeficiency syndrome (AIDS), a disease, by which over 25 million people were killed in the past three decades. The major difficulty with HIV is the extremely high mutation rate of the viral genome leading to an enormous diversity of the HIV proteome, and allowing the virus to escape the human immune response. Conventional consensus based peptide pools address this problem only very poorly. Therefore, when designing the new HIV-1 ENV, GAG, NEF and POL Ultra PepMix™ Peptide Pools, we focused primarily on coverage of the tremendous sequence diversity of HIV. Protein sequences of all subtypes of HIV-1/SIVcpz from the Los Alamos National Lab were considered. We developed a proprietary algorithm to obtain peptide combinations providing optimal coverage of the protein variability. Broad clade coverage provided by these new peptide pools enables stimulation and monitoring of immune responses in HIV patients independent of geographical origin.
Benefits of PepMix™
- Equivalent or better stimulation of CD4+ and CD8+ T-cell responses compared to whole protein antigens
- Simultaneous detection of CD4+ and CD8+ responses in a single sample
- Improved responses in stored blood and PBM cells compared to whole protein antigens
- More reliable as control than phytohemagglutinin (PHA) and concanavalin A (ConA)
- High-batch-to-batch reproducibility
- Reliable chemical and biochemical quality control and quality assurance for peptide synthesis and peptide pool generation
- Prolonged shelf stability when stored freeze dried
Selected References
"In Vitro Studies of the Impact of Maribavir on CMV-Specific Cellular Immune Responses"
Stachel et al., J Clin Virol. (2015) - PMID: 26780109
"Induction Of Broad-based Immunity and Protective Efficacy by Self-amplifying mRNA Vaccines Encoding Influenza Virus Hemagglutinin"
Brazzoli et al., Journal of Virology (2015) - PMID: 26468547
"Targeting of Nucleoprotein to Chemokine Receptors by DNA Vaccination Results in Increased CD8+-mediated Cross Protection Against Influenza"
Baranowska et al., Vaccine (2015) - PMID: 26387432
"Characteristics of Immune Memory 10-15 years After Primary Hepatitis B Vaccination"
Hummel et al., Vaccine (2015) - PMID: n.a.
"T Cells Specific for Different Latent and Lytic Viral Proteins Efficiently Control Epstein-Barr Virus Transformed B Cells"
Nowakowska et al., Cytotherapy (2015) - PMID: 26276009
"High-density Preculture of PBMCs Restores Defective Sensitivity of Circulating CD8 T Cells to Virus- and Tumor-derived Antigens"
Wegner et al., Blood (2015) - PMID: 26024876
"Metabolic Regulation of Hepatitis B Immunopathology by Myeloid-derived Suppressor Cells"
Pallett et al., Nature Medicine (2015) - PMID: 25962123
Application Notes
"Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy"
Lam et al. (2015) Full text
"Peptide-stimulated Expansion of Virus-specific T cells For Preventative Treatment After Allogeneic Stem Cell Transplantation"
Gary et al. (2015) Full text
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Testimonials
"To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D . Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids. Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our "go-to" company for purchasing peptides."
Dr. Laura J Pallett, Infection and Immunity, University College London, UK
| Application | Antigen specific T-cell stimulation Immune monitoring T-cell assays T-cell expansion |
| Protein Name | Nef protein (NEF) |
| Organism | Human immunodeficiency virus (HIV) |
| Indication / Topic | AIDS (HIV) |
| Number of peptides | 150 |
| Amount | 1 vial containing 15 nmol (appr. 25µg) per peptide |
| Specifications | Selected immunogenic peptides (15mers) |
| Documentation | Protocol, Datasheet and Annotations |
| Sequence | See Datasheet under Product Documentation |