产品资料

Diapharma/Chromogenix Coatest® APC™ Resistance – V/K823120/Kit/80-160 tests

Description:

ChromogenixCoatest®APC™Resistance–VisanAPTT-basedassaykitforthescreeningoffactor-V-relatedAPCresistance.ThehighsensitivityandspecificityofthetestforthefactorV:Q506mutationisobtainedbypredilutingthesampleplasmawithanexcessofV-DEFPlasmabioreagent.ThetestdesignmakesitpossIBLetodiscriminatebetweenheterozygousandhomozygousfactorVgenotypes.Italsoallowsforanalysisofplasmafrompatientsonheparinororalanticoagulanttherapy.Highdiscriminationbetweengenotypeswith100%sensitivityforFV:Q506.ReducesneedforPCRdetermination.Applicabletoanticoagulanttreatedpatients.

Atime-andmoney-savingalternativetoindividualgenetictesting,Coatest©APC™Resistancetestingisanidealsolutionforphysiciansandlabs.Eachkitwasdesignedtobeeasytouseforin-housescreening,offeringfastresultsandlowcostsforbothlarge-andsmall-volumelabs.ThemostcommonFVLeidenscreeningtestperformed,itoffersunmatchedsensitivityfortheFV:Q506mutation–closeto100%specificity–andisapplicabletopatientsonheparinorwarfarin.

MeasurementPrinciple:

OnevolumeofplasmaispredilutedwithfourvolumesofV-DEFPlasma.ThedilutionisthenincubatedwiththeAPTTreagentforastandardperiodoftime.CoagulationistriggeredbytheadditionofCaCl2intheabsenceandpresenceofexogenousAPCandthetimeforclotformationisrecorded.

ActivatedProteinC(APC)isaregulatorofthecoagulationcascade,byspecificallyinactivatingfactorsVaandVIIIa,inthepresenceofphospholipidsandcalcium. Inmostofthecases(morethan90%),ActivatedProteinCResistance(APCR)phenotypeiscausedbyaFactorVgenemutation(FactorVLeiden).Themutation,locatedonFactorVexon10(1691G–>A),ofargininetoglutamineonposition506,rendersFactorVaresistanttothecleavagebyActivatedProteinC.ThisgeneticanomalycanbeevidencedwithaclottingmethodperformedinthepresenceortheabsenceofActivatedProteinC.

KitComposition:

apc resistance v leiden clotting assay test kit

ReagentsandtheirstABIlitywhenopened

Reagent	Size	Stability	Temp
APC/CaCl₂	4×2ml	5days 8hours	2-8°C 15-25°C
APTTreagent	1×16ml	1week 1month	15-25°C 2-8°C
CaCl₂	1×8ml	1month 1week	2-8°C 15-25°C
V-DEFPlasma	4×4ml	8hours 24hours 3months	15-25°C 2-8°C -20°C
APC/CaCl₂	4×2ml	3months	-20°C
ControlPlasmaLevel1	1×1ml	6hours 3months	2-25°C -20°C
ControlPlasmaLevel2	1×1ml	6hours 3months	2-25°C -20°C

Determinationsperkit:80–160

FactorVLeidenTestingAlgorithm:

IDEALFOR:

Labslookingtobringtestingin-house
Labslookingforcostsavings
Orderingphysicianslookingforasensitiveassaywithrapidturnaround
Savesthelabfromhavingtoperformgenetictestonallpatients
Economicalalternativetogenetictesting

FV:Q506MutationScreening:

FV:Q506 mutation screening apc resistance assay test kit

TheChromogenixCoatestAPCResistanceVmethodwasusedforanalysisofplasmafromvarioussamplecategoriesshowingdiscriminationbetweennormalandmutatedfactorVgenotypes.

OAC=OralAnticoagulantTherapy

Background:

ActivatedProteinC(APC)isaregulatorofthecoagulationcascade,byspecificallyinactivatingfactorsVaandVIIIa,inthepresenceofphospholipidsandcalcium.

Inmostofthecases(morethan90%),ActivatedProteinCResistance(APCR)phenotypeiscausedbyaFactorVgenemutation(FactorVLeiden).Themutation,locatedonFactorVexon10(1691G–>A),ofargininetoglutamineonposition506,rendersFactorVaresistanttothecleavagebyActivatedProteinC.ThisgeneticanomalycanbeevidencedwithaclottingmethodperformedinthepresenceortheabsenceofActivatedProteinC.

ActivatedProteinCResistanceistestedbyusingaclottingmethodperformedwithorwithoutActivatedProteinC.

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