Diapharma/Technoclone Anti-tPA Monoclonal Antibody 3VPA/TC21023/Vial/500µg

Description:

TechnocloneAnti-tPAMonoclonalAntibody3VPA

IgG₁;halfmaximalbindingtocoatedt-PAat8.5µg/mL(highaffinity);Reactionwithanepitopeexpressedonboththefingerdomainandgrowthfactordomainoft-PA.Competitiveinhibitionofplasminogenactivationinthepresenceoffibrin(Ki=0.68nM). ItcanbeusedinanELISA-systemassecondantibody.

Specificity

BindstotPA.ReactionwithanepitopeexpressedonboththefingerdomainandgrowthfactordomainoftPA(1).Halfmaximalbindingtocoatedt-PAAT8.5µg/ml(highaffinity).Competitiveinhibitionofplasminogenactivationinthepresenceoffibrin(Ki=0.68nM).

Derivation

HybridizationofNSOcellswithspleencellsfromaBALB/cmouseimmunizedwithpurifiedmelanoma-typet-PA.

Antibodyclass

IgG₁

Purification

TheantibodyispurifiedfrommurineascitesbyaffinitychromatographyonimmobilizedrecombinantProteinA.ThepurityoftheproductistestedbyLaemmliSDS-PAGE.

Handlingandstorage:

Theantibodyislyophilizedfroma1mg/mlsolutioninisotonicphosphatebufferedsaline,pH7.4,containing0.02%sodiumazideand20mg/mlmannitol.Itissuppliedinvialsof500µgandshouldbereconstitutedwith0.5mldistilledwater.Forextensivedilutionsaproteincontainingsolutionshouldbeused(e.g.1%bovineserumalbumininPBS).Lyophilzedantibodyshouldbestoredat4°C.Reconstitutedantibodyshouldbealiquotedandstoredat-20°Corlower.Avoidrepeatedfreeze-thawcycles.

Literature:

1)J.Wojta,L.Turcu,O.F.Wagner,C.Korninger,B.R.Binder:Evaluationoffibrinolyticcapacitybyacombinedassaysystemfortissue-typeplasminogenactivatorantigenandfunctionusingmonoclonalanti-tissue-typeplasminogenactivatorantibodies.J.Lab.ClinMed.105:665.671,1987.2)J.Wojta,R.L.Hoover,andT.O.Daniel:VascularOriginDeterminesPlasminogenActivatorExpressioninHumanEndothelialCells.J.Biol.Chem.264(5):2846,1989.