Diapharma/Technoview® UFH Calibrator Kit/5090071/Box/2x1ml

Description:

Technoview®UFHCalibratorKit includesplasma-derivedcalibration sampleswithunfractionatedheparin(UFH)concentrationsof ~0IU/mland~1.4IU/ml. Thecalibrators canbeusedfor establishingastandardcurveinresearchmeasurementsofheparin.Theyare optimizedforusewithanti-Xachromogenicassaysandare frequentlyusedinconjunctionwiththe Technoview®UFH lowandhighcontrolsinthe Technochrom®anti-Xachromogenicassay.

Advantages:

• Allowsforestablishmentofacalibrationcurvefor unfractionatedheparin
• Optimizedforusewithanti-Xa(withoutexogeneousantithrombin)research assays

CalibratorKitComposition:

2x1mlvialswithunfractionatedheparin (UFH) concentrations:

• Cal1:0IU/ml
• Cal5:1.4IU/ml

Calibratorsare lyophilizedandpreparedfrompooledhumancitratedplasmasupplementedwithdifferent concentrations(ornone)ofunfractionatedheparin.TheplasmacontainsstABIlizersbutnobactericideadditives.Theyare calibratedagainstthe5thInternationalStandard preparationforUFH97/578.

Background:

HeparinisawidelyusedantithromboticagentthatcanbeadmiNISTeredviaanintravenousorsubcutaneousroute.Itisnaturallyoccurringandtypicallyderived from bovineorporcinetissue. Theanticoagulanteffect ofthissulfatedglycosaminoglycanresultsfromitsbindingtoantithrombin(AT),themajorinhibitorofthecoagulationcascade.Bindinginducesaconformationalchange intheAT molecule,whichgreatlyacceleratestheAT inhibitionofseveralcoagulation proteases.TheacceleratedinhibitionofthrombinandFactorXabyheparinistheprimarybasisforitsanticoagulationproperties.

Twoformsofheparinareusedtherapeutically:unfractionatedheparin(UFH),withanaveragemolecularweight(MW)of15,000andlowmolecularweightheparin(LMWH),withaMWbetween4,000and6,500.ThelowerMWresultsinLMWHactingprimarilyonFactorXa,whereasUFHefficientlyinhibitsboththrombinandFactorXa. Bothheparinformsshouldbemonitored.UFHcanbemeasured withanaPTTorananti-XachromogenicassaywhileLMWHshouldbemeasuredusingandanti-Xachromogenicassay.NotethatthiscalibratorkitisforresearchuseonlyintheUSandCanada.