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Discovery Antibodies/Anti-KV1.3 (KCNA3) (extracellular)-FITC Antibody/10 x 50 µl/APC-101-F-10 x 50 µl188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Discovery Antibodies/Anti-KV1.3 (KCNA3) (extracellular)-FITC Antibody/10 x 50 µl/APC-101-F-10 x 50 µl

Cat#:APC-101-F
AlternativeNamePotassiumvoltage-gatedchannelsubfamilyAmember3
  • LyophilizedPowder
    • Type:Polyclonal
    Host:Rabbit
    Reactivity:h,m,r
      • PeptideKDYPASTSQDSFEA(C),correspondingtoaminoacidresidues263-276ofhumanKV1.3(AccessionP22001).ExtracellularloopbetweendomainsS1andS2.
        Anti-KV1.3 (KCNA3) (extracellular)-FITC Antibody
    Accession(Uniprot)NumberP22001
    GeneID3738
    PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
    HomologyRat,mouse -12/14aminoacidresiduesidentical.
    RRIDAB_2040147.
    PurityAffinitypurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
    IsotypeRabbitIgG.
    LabelFluoresceinisothiocyanate(FITC).
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution50µldoubledistilledwater(DDW).
    Antibodyconcentrationafterreconstitution1mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).
    StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#APC-101),anddirectflowcytometry(labeledantibody).
    Applications:fc,ic,if,lci
      • Anti-KV1.3 (KCNA3) (extracellular)-FITC Antibody
        CellsurfacedetectionofKV1.3inliveintactJurkat(humanTcellleukemia)cells:
        ___ Unstainedcells.
        ___ Cells + Anti-KV1.3(KCNA3)(extracellular)-FITC Antibody(#APC-101-F),(0.5-5µgantibody/1x106 cells).
        Thecontrolantigenisnotsuitableforthisapplication.
      • Mouselivingprimarycorticalcells(Duque,A. etal. (2013) Develop.Neurobiol. 73, 841.).
    References
    • 1.Chandy,K.G.etal.(2001)Toxicon39,1269.
    • 2.Koo,G.C.etal.(1997)J.Immunol.158,5120.
    • 3.Grissmer,S.etal.(1994)Mol.Pharmacol.45,1227.
    • 4.Garcia-Calvo,M.etal.(1993)J.Biol.Chem.268,18866
    • 5.Garcia,M.L.etal.(1994)Biochemistry33,6834.
    • 6.Koschak,A.etal.(1998)J.Biol.Chem.273,2639.
    • 7.Kalman,K.etal.(1998)J.Biol.Chem.273,32697.

      • KV1.3 belongstothe Shaker familyof voltage-dependentK+ channels.Thechannel,encodedby KCNA3,iswidelyexpressedinthebrain,lungandosteoclastsandinseveralcell populationsofhematopoieticorigin.TheprominenceofKV1.3channelsinthesecells(particularlyinTlymphocytes)directedmuchresearch attention.ItwasfoundthatKV1.3isthemainchannelresponsIBLeformaintainingtherestingpotentialinquiescentcellsandregulatingthe Ca2+ signalingthatisindispensablefornormalTlymphocyteactivation.1,2 BasedonthecentralroleofKV1.3inregulatingtheinitiationofanimmuneresponse, thechannelhasbeenrecognizedasapotentialtargetforimmunosuppressantdrugs.1

        KV1.3channelsarepotentlyinhibitedbyseveralvenomouspeptidetoxinsamongthem CharyBDotoxin (2.6nM), Noxiustoxin (1nM), Kaliotoxin (0.65nM), Margatoxin (0.05nM), Agitoxin-1 (1.7nM), Agitoxin-2 (0.004nM), Hongotoxin-1 (0.09nM) and Stichodactylatoxin (0.01nM).3-7

        ThecentralroleofKV1.3inimmunesystemcellscreatedarealneedforaspecific antibodythatwouldbeabletoworkinflowcytometryapplications.

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IF-Immunofluorescence,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    LyophilizedPowder
    Lastupdate:16/01/2020

    Anti-KV1.3(KCNA3)(extracellular)Antibody(#APC-101)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanprotein.Theantibodycanbeusedinwesternblot,immunohistochemistry,livecellimaging,andindirectflowcytometryapplications.IthasbeendesignedtorecognizeKV1.3potassiumchannelfromhuman,mouse,andratsamples.

    Anti-KV1.3(KCNA3)(extracellular)-FITCAntibody(#APC-101-F)isdirectlyconjugatedtofluoresceinisothiocyanate(FITC).Thislabeledantibody canbeusedinimmunofluorescentapplicationssuchasdirectflowcytometryandlivecellimaging.

    Forresearchpurposesonly,notforhumanuse
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