RNasin® Ribonuclease Inhibitor

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不抑制SP6, T7 或T3 RNA聚合酶; GoScript™ 逆转录酶, AMV或M-MLV 逆转录酶; 或Taq DNA 聚合酶。 可用于多种的下游检测: 在很宽的pH范围内(pH5-8)广谱抑制真核细胞RNase(pH 5-8)。 在很宽的温度范围内都可保持抑制活性。
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This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab\'s needs.

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Add to HelixCloseThis product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab\'s needs.

Learn more about the Helix Collection

Add to HelixCloseThis product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab\'s needs.

Learn more about the Helix Collection

Add to HelixCloseThis product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab\'s needs.

Learn more about the Helix Collection

Add to HelixCloseThis product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab\'s needs.

Learn more about the Helix Collection

Add to HelixCloseThis product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab\'s needs.

Learn more about the Helix Collection

在过去的25年里，RNasin® 抑制剂已经在超过10,000篇文章中出现，是世界范围内最广泛应用的RNA酶抑制剂。

RNasin可抑制RNA酶A, B和C对RNA的降解，让您可安心处理重要样本来源的RNA。

RNasin® RNA酶抑制剂可用于RNA提取, RT-PCR, 体外转录或任何RNA酶成为影响因素的情况。

我们提供了天然型和重组型RNAsin®抑制剂, 同时还提供RNAsin® Plus—一种加强型的RNA酶，抗氧化能力更强，热稳定性高达70°C。

View RNasin Citations
Inhibits RNase A, B and C as well as human placental RNase. Does not inhibit other RNases and modifying enzymes like reverse transcriptases. Results in higher yield in RNA extractions and better performance in RT-PCR, cDNA synthesis, microarrays and in vitro transcription/translation.
Possesses all the proven features of Recombinant RNasin Ribonuclease Inhibitor. Stable against oxidation and heat, leading to higher stability up to 15 minutes at 70 C. Particularly suitable for long-term storage of samples.

RNases are ubiquitous and can cause RNA degradation and compromise RNA integrity. RNase contamination can happen during RNA purification and during routine downstream handling. RNasin® Ribonuclease Inhibitors inhibit RNase A family and human placental RNases by noncovalently binding to RNases.

In this experiment, nine buffer and water stock solutions from an RNA-free zone of an academic lab were tested for RNases. The samples were in use for 1-14 months. Thirty-five microliters of each sample was collected, split and mixed with 5 µg of RNA and RNase ONE™ 10X buffer. RNasin® Ribonuclease Inhibitor (40u) was added to one of the two samples, and both were incubated overnight at 37 °C. Lane 1, marker; lane 2, RNA control; lanes 3–11, samples from the laboratory.

Of the nine samples tested, seven showed varying degrees of RNase contamination. For all samples tested, the RNA remained intact during the overnight incubation at 37°C when Recombinant RNasin® Ribonuclease Inhibitor was added. Details of this experiment can be found in this technical article.

Some RNase inhibitors offer less than half the protection of Promega RNasin Inhibitor.

Here, we compare RNasin Ribonuclease Inhibitor and RNaseOUT for inhibition of RNase A during RT-qPCR. RNA was reverse transcribed and amplified in the presence of RNase A and either Promega RNasin or RNaseOUT . RNasin Inhibitor provided better RNA protection in the presence of RNase, as shown by higher levels of mRNA.

Establish an RNA Only Work Zone in your lab that has dedicated equipment, reagents, labware, etc. Use these tips to help you avoid RNase contamination.

Wear gloves and use sterile technique when handling RNA or reagents that will be used with RNA. The most common sources of RNase contamination are hands and bacteria or mold that could be lurking on dust particles or on glassware. Use sterile, disposable plasticware. These products are typically RNase-free and don t require treatment to inactivate RNases. Treat non-disposable glassware and plasticware before you use it. For glassware, bake it at 250 C overnight. For plasticware, rinse it with 0.1N NaOH/1mM EDTA and then with diethyl pyrocarbonate (DEPC)-treated water. Reserve a set of chemicals and solutions needed for RNA isolation and analysis as RNA Only and keep them separate from reagents for other applications. Wear gloves whenever you handle these reagents, and use sterile labware to measure them. Beware! Autoclaving alone is not sufficient to inactivate all RNases. Solutions that you prepare in the lab should be treated by adding DEPC to 0.05% and incubated overnight. Autoclaving the treated solutions for 30 minutes will remove any trace of the DEPC from the solution. Use RNasin Ribonuclease Inhibitor to help prevent RNA degradation.

Recombinant and RNasin Plus: Recombinant E. coli (Cat.# N2511, N2515, N2611, N2615).

Native: Human placenta (Cat.# N2111, N2115).

One unit is defined as the amount of RNasin® Ribonuclease Inhibitor required to inhibit the activity of 5ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2,3´-cyclic monophosphate by ribonuclease A.

Use Restrictions For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Use Restrictions For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Use Restrictions For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
Use Restrictions For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

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