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Millipore/MAB3510P | Anti-BrdU Antibody, clone BU-1, Peroxidase conjugated/MAB3510P/100 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Millipore/MAB3510P | Anti-BrdU Antibody, clone BU-1, Peroxidase conjugated/MAB3510P/100 µg

Description
CatalogueNumberMAB3510P
BrandFamilyChemicon®
TradeName
  • Chemicon
DescriptionAnti-BrdUAntibody,cloneBU-1,Peroxidaseconjugated
AlternateNames
  • BrdU
BackgroundInformationBromodeoxyuridine(BrdU)isathymidineanalogandisspecificallyincor-poratedintoDNAduringDNAsynthesis.Anti-bromodeoxyuridinemonoclonalantibodyisusedtoidentifycellsthathaveincorporatedBrdU.ThisimmunologicaldetectionschemehasseveraladvantagesovertheuseofrADIoactivethymidineincorporationforidentifyingcellsunder-goingreplication.Labelinganddetectioncanbeperformedthesamedayinsteadofwaitingseveraldays,asrequiredforautoradiographyoftritium-labeledcells,andthenecessityofusingmultiplespecimensforobtainingtheoptimalexposuretimeiseliminated.
ProductInformation
FormatHRP
PresentationLyophilizedpurifiedHRPconjugatedIgG.Buffer=10mMPBScontaining0.1%thimerosaland15mg/mlBSA.Reconstititutewith100microlitersofsteriledistilledwater.PossIBLemycoplasmariskcontainedwithinthisproduct.Useappropriatemeasurestopreventcelllineinfection/transmission.
StorageandShippingInformation
StorageConditionsMaintainat2-8°Cinundilutedaliquotsforupto6months.Avoidfreezingafterreconsituition.
Applications
ApplicationAnti-BrdUAntibody,cloneBU-1,PeroxidaseconjugatedisaMouseMonoclonalAntibodyfordetectionofBromodeoxyuridinealsoknownasBrdU&hasbeenvalidatedinFC,ICC&IHC.
KeyApplications
  • FlowCytometry
  • Immunocytochemistry
  • Immunohistochemistry
ApplicationNotesImmunocyto/histochemistry:1:300

DilutionbuffersshouldnotcontainsodiumazidesinceitinhibitsHRPactivity.

Optimalworkingdilutionsmustbedeterminedbyenduser.
BIOLOGicalInformation
ImmunogenBromodeoxyuridine
Epitopenoacidorproteinaserequired
CloneBU-1
ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
HostMouse
SpecificityTheantibodyspecificallybindstobromodeoxyuridineandcrossreactswithiodouridine(10%).Anti-bromodeoxyuridinedoesnotcrossreactwithfluorodeoxyuridine,norwithanyendogenouscellularcomponentssuchasthymidineoruridine.MAB3510PdoesnotrequirepretreatmentofcellswithstrongDNAdenaturantsinorderfortheantibodytorecognizeincorporatedBrdU.
IsotypeIgG2a
SpeciesReactivity
  • All
AntibodyTypeMonoclonalAntibody
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
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