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Millipore/06-1319 | Anti-Histone H2A.Bbd Antibody/06-1319/100 µg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Millipore/06-1319 | Anti-Histone H2A.Bbd Antibody/06-1319/100 µg

Description
CatalogueNumber06-1319
DescriptionAnti-HistoneH2A.BBDAntibody
AlternateNames
  • H2Ahistonefamily,memberB1
  • H2ABarrbody-deficient
  • histoneH2A-Bbdtype1
BackgroundInformationHistoneH2A.Bbd(Barrbody-deficient),alsoknownashistoneH2A-Bbdtype1,isanatypicalhistonevariant.ThishistonevariantassociateswithH2BandreplaceshistoneH2Ainthenucleosome.IncontrasttootherH2Ahistones,thisvariantlacksconservedresiduesthataretargetsofpost-translationalmodifications.ConsequentlythisH2A.Bbdvariantisabout15aminoacidsshorterandonly48%similartothecanonicalH2A.ThemajordifferencesbetweenthetwoarefoundinthedockingdomainthatlinksH2A-H2BtotheH3-H4tetramer.Nucleosomescontainingthishistoneadoptamorerelaxedstructureandprotect~118basepairsofDNAinsteadofthe147basepairsfoundinclassicalnucleosomes.ThisvarianthistoneisassociatedwithactiveXchromosomesandautosomes,andistypicallyabsentfrominactiveXchromosomes.
ProductInformation
FormatAffinityPurified
Control
  • CelllysateoverexpressingH2A.Bbd.
PresentationPurifiedrabbitpolyclonalinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide.
StorageandShippingInformation
StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.
Applications
ApplicationUseAnti-HistoneH2A.BbdAntibody(RabbitPolyclonalAntibody)validatedinWBtodetectHistoneH2A.BbdalsoknownasH2AhistonefamilymemberB1,H2ABarrbody-deficient.
KeyApplications
  • WesternBlotting
BIOLOGicalInformation
ImmunogenKLH-conjugatedlinearpeptidecorrespondingtohumanHistoneH2A.Bbd.
ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
HostRabbit
SpecificityThisantibodyrecognizesHistoneH2A.Bbd.
SpeciesReactivity
  • Human
  • Mouse
AntibodyTypePolyclonalAntibody
EntrezGeneNumber
EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneencodesamemberofthehistoneH2Afamily.ThisgeneispartofaregionthatisrepeatedthreetimesonchromosomeX,onceinintron22oftheF8geneandtwiceclosertotheXqtelomere.Thisrecordrepresentsthemostcentromericcopywhichisinintron22oftheF8gene.[providedbyRefSeq].
GeneSymbol
  • H2A.Bbd
  • H2AFB1
PurificationMethodAffinityPurfied
UniProtNumber
UniProtSummaryFUNCTION:AtypicalhistoneH2AwhichcanreplaceconventionalH2Ainsomenucleosomes.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.Nucleosomescontainingthishistonearelessrigidandorganizeonly118basepairofDNAinsteadof147inclassicalnucleosomes.TheyareassociatedwithtranscriptionallyactivechromatinandexcludedformBarrbodies.Ref.4Ref.5

SUBUNITSTRUCTURE:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Maybeincorporatedintoaproportionofnucleosomes,replacingoneormoreH2Amolecules.

SUBCELLULARLOCATION:Nucleus.Chromosome.Note:AssociatedwiththeactiveXchromosomeandwithautosomes,whileitisabsentfromtheinactiveXchromosomeandexcludedfromBarrbodies.

DOMAIN:ThedockingdomainisresponsiblefortheweakerheterodimerizationwithH2B.Ref.4

MISCELLANEOUS:IncontrasttootherH2Ahistones,itdoesnotcontaintheconservedresiduesthatarethetargetofpost-translationalmodifications.

SEQUENCESIMILARITIES:BelongstothehistoneH2Afamily.
MolecularWeight~17kDaobserved
PhysicochemicalInformation
Dimensions
MaterialsInformation
MaterialsInformation
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