Description | |
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CatalogueNumber | 06-1319 |
Description | Anti-HistoneH2A.BBDAntibody |
AlternateNames |
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BackgroundInformation | HistoneH2A.Bbd(Barrbody-deficient),alsoknownashistoneH2A-Bbdtype1,isanatypicalhistonevariant.ThishistonevariantassociateswithH2BandreplaceshistoneH2Ainthenucleosome.IncontrasttootherH2Ahistones,thisvariantlacksconservedresiduesthataretargetsofpost-translationalmodifications.ConsequentlythisH2A.Bbdvariantisabout15aminoacidsshorterandonly48%similartothecanonicalH2A.ThemajordifferencesbetweenthetwoarefoundinthedockingdomainthatlinksH2A-H2BtotheH3-H4tetramer.Nucleosomescontainingthishistoneadoptamorerelaxedstructureandprotect~118basepairsofDNAinsteadofthe147basepairsfoundinclassicalnucleosomes.ThisvarianthistoneisassociatedwithactiveXchromosomesandautosomes,andistypicallyabsentfrominactiveXchromosomes. |
ProductInformation | |
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Format | AffinityPurified |
Control |
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Presentation | Purifiedrabbitpolyclonalinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide. |
StorageandShippingInformation | |
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StorageConditions | Stablefor1yearat2-8°Cfromdateofreceipt. |
BIOLOGicalInformation | |
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Immunogen | KLH-conjugatedlinearpeptidecorrespondingtohumanHistoneH2A.Bbd. |
Concentration | PleaserefertotheCertificateofAnalysisforthelot-specificconcentration. |
Host | Rabbit |
Specificity | ThisantibodyrecognizesHistoneH2A.Bbd. |
SpeciesReactivity |
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AntibodyType | PolyclonalAntibody |
EntrezGeneNumber | |
EntrezGeneSummary | HistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneencodesamemberofthehistoneH2Afamily.ThisgeneispartofaregionthatisrepeatedthreetimesonchromosomeX,onceinintron22oftheF8geneandtwiceclosertotheXqtelomere.Thisrecordrepresentsthemostcentromericcopywhichisinintron22oftheF8gene.[providedbyRefSeq]. |
GeneSymbol |
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PurificationMethod | AffinityPurfied |
UniProtNumber | |
UniProtSummary | FUNCTION:AtypicalhistoneH2AwhichcanreplaceconventionalH2Ainsomenucleosomes.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.Nucleosomescontainingthishistonearelessrigidandorganizeonly118basepairofDNAinsteadof147inclassicalnucleosomes.TheyareassociatedwithtranscriptionallyactivechromatinandexcludedformBarrbodies.Ref.4Ref.5 SUBUNITSTRUCTURE:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Maybeincorporatedintoaproportionofnucleosomes,replacingoneormoreH2Amolecules. SUBCELLULARLOCATION:Nucleus.Chromosome.Note:AssociatedwiththeactiveXchromosomeandwithautosomes,whileitisabsentfromtheinactiveXchromosomeandexcludedfromBarrbodies. DOMAIN:ThedockingdomainisresponsiblefortheweakerheterodimerizationwithH2B.Ref.4 MISCELLANEOUS:IncontrasttootherH2Ahistones,itdoesnotcontaintheconservedresiduesthatarethetargetofpost-translationalmodifications. SEQUENCESIMILARITIES:BelongstothehistoneH2Afamily. |
MolecularWeight | ~17kDaobserved |
PhysicochemicalInformation |
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Dimensions |
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MaterialsInformation |
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MaterialsInformation |
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