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| ProductName | HIF-1aNTAD(Hypoxia-InducIBLeFactor1alphaN-TerminalActivationDomain(530-698) |
| Size | 10,000U |
| Description | Hypoxia-induciblefactor-1(HIF1)isatranscriptionfactorfoundinmammaliancellsculturedunderreducedoxygentensionthatplaysanessentialroleincellularandsystemichomeostaticresponsestohypoxia.HIF1isaheterodimercomposedofanalphasubunitandabetasubunit.Thebetasubunithasbeenidentifiedasthearylhydrocarbonreceptornucleartranslocator(ARNT).ThisgeneencodesthealphasubunitofHIF-1.HIF-1alphacontainstwotransactivationdomainslocatedbetweenaminoacids531and826.Overexpressionofanaturalantisensetranscript(aHIF)ofthisgenehasbeenshowntobeassociatedwithnonpapillaryrenalcarcinomas.Twoalternativetranscriptsencodingdifferentisoformshavebeenidentified.SpecificdisruptionoftheHIF-1pathwayisimportantforexploringitsroleintumorBIOLOGyanddevelopingmoreefficientweaponstotreatcancer(1).HIF-1alphaisamasterregulatorofthehypoxicresponse,anditsproangiogenicactivitiesinclude,butarenotlimitedto,regulationofvascularendothelialgrowthfactor(VEGF)(2).HIF-1alphaproteinexpressionoftenseenininvasivebreastcancer(3).RecentdatademonstratesthatHIF-1alphaknockdownreducestumOrigenicityofMCF-7cellsandsuggestapromisingcombinationofbothanti-HIF-1strategyandtrADItionalchemotherapytoimprovecancertreatment(4) |
| Applications |
|
| Source | E.coli |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 3091 |
| Accession# | NM_001530 |
| GeneName | hypoxiainduciblefactor1,alphasubunit(basichelix-loop-helixtranscriptionfactor) |
| GeneSymbol | HIF1A |
| GeneSynonyms | HIF1;MOP1;PASD8;HIF-1A;bHLHe78;HIF-1alpha;HIF1-ALPHA |
| MW | 19.8kDa |
| ProteinSequence | EFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTT |
| StorageBuffer | 20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Semenza,G.L.etal.,(1994)J.Biol.Chem.269(38),23757-23763 2.Corley,K.M.etal.,(2005)J.Cell.Biochem.96(5),971-985 3.Vleugel,M.M.,etal.,(2005)CancerGenet.Cytogenet.163(2),168-172 4.Li,J.,etal.(2006)Biochem.Biophys.Res.Commun.342(4),1341-1351 |
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