Description | E.coliDNAligasecatalyzestheformationofaphosphodiesterbondbetweenanadjacent5'phosphateanda3'hydroxylofDNAends.TheenzymecanbeusedfortheligationofdoublestandedDNAcontainingcohesiveends.Itshouldnotbeusedforbluntendligationandnicksealing. |
Applications | OkayamaandBergCDNAcloning |
Source | PurifiedfromarecombinantE.colistraincarryingtheE.coliDNALigasegene |
Components |
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UnitDefinition | 1unitisdefinedastheamountofE.coliDNALigaserequiredtoligate50%of100ngDNAfragmentswithcohesiveterminiin30minutesat25°C. |
QualityControl |
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Storage | -20°C. |
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