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Excellgen/E. coli DNA Ligase/EG-1049/2,500 Units188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Excellgen/E. coli DNA Ligase/EG-1049/2,500 Units

Description

E.coliDNAligasecatalyzestheformationofaphosphodiesterbondbetweenanadjacent5'phosphateanda3'hydroxylofDNAends.TheenzymecanbeusedfortheligationofdoublestandedDNAcontainingcohesiveends.Itshouldnotbeusedforbluntendligationandnicksealing.

ApplicationsOkayamaandBergCDNAcloning
SourcePurifiedfromarecombinantE.colistraincarryingtheE.coliDNALigasegene
Components
  • Ecoli.DNALigase,10,000U/mlinstoragebuffer:10mMTris-HCl,pH7.5@25°C,50mMKCl,1mMDTT,0.1mMEDTA,50%glycerol
  • 10XE.coliDNALigaseBuffer:300mMTris-HCl,pH8@25°C,40mMMgCl2,260μMNAD,10mMDTT,500μg/mlBSA.
UnitDefinition1unitisdefinedastheamountofE.coliDNALigaserequiredtoligate50%of100ngDNAfragmentswithcohesiveterminiin30minutesat25°C.
QualityControl
  • Theabsenceofendo-,exodeoxyribonucleases,phosphatases,andribonucleasesconfirmedbyappropriatequalitytests.
  • Functionallytestedforthecapacitytojoincohesive-andblunt-endDNAfragments.
Storage-20°C.
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