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Excellgen/Klenow Fragment (3->5 exo-)/EG-1056/2,500 Units188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Excellgen/Klenow Fragment (3->5 exo-)/EG-1056/2,500 Units

Description

KlenowFragment(3´→5´exo-)isanN-terminaltruncationofDNAPolymeraseIwhichretainspolymeraseactivity,buthaslostthe5´→3´exonuclease(nick-translation)activity,andproofreADIng(3′→5′)exonucleaseactivityduetotwoaminoacidsmutations(D355A,E357A).

Applications
  • Random-primedDNAlabeling
  • DNAsequencingbytheSangerdideoxymethod
  • SecondstrandCDNAsynthesis
  • Labelingbyfill-in5'-overhangsofdsDNA
  • Stranddisplacementamplification(SDA)
SourceArecombinantE.colistraincarryingthemutatedKlenowFragmentofEcoliPolAgene.
UnitDefinition

Oneunitisdefinedastheamountofenzymethatwillincorporate10nmolofdNTPintoacidinsolublematerialin30minutesat37°C.

Components
  • KlenowFragmentexo-:5,000U/mlin25mMTris-HCl,1mMDTT,0.1mMEDTA,50%Glycerol,pH7.4@25°C
  • 10XReactionBuffer:500mMNaCl,100mMTris-HCl,100mMMgCl2,10mMDTT,pH7.9@25°C
QualityControl
  • Theabsenceofendo-andexodeoxyribonucleasesconfirmedbyappropriatequalitytests.
  • Functionallytestedinrandom-primedDNAlabeling.
StorageCondition

-20°C

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