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Glen research/Glen UnySupport™ 1000/1kit/20-5141-45

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Description
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Intellectual Property
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Technical Documents

Safety Data Sheet

Glen Report 20.25: New Universal Support - Glen UnySupport

Glen Report 10.15: Universal Support Replaces Individual Columns

Description

Our original Universal Support (20-5000) has been discontinued since complete dephosphorylation using ammonium hydroxide, AMA or anhydrous methylamine gas takes longer than most companies wish to allocate.A recent development has been the use of a support based on a molecule which is "conformationally preorganized" to accelerate the dephosphorylation reaction.1,2By using a rigid bicyclic molecule on the support, the rate of elimination is markedly faster than the original Universal Support.The structure of Glen UnySupport is shown below.The N-phenyl version, developed at Isis Pharmaceuticals as UnyLinker™, is available from several companies for large scale oligo synthesis.Glen UnySupport is the N-methyl version, which is preferred for high throughput oligonucleotide synthesis since methylamine rather than aniline is formed on deprotection.We are happy to offer Glen UnySupport in a variety of popular formats under license from Isis Pharmaceuticals.

Details

Usage

  • Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
  • Deprotection: Solution phase:Ammonium Hydroxide:MethylAmine (AMA) 1:1 for 1 hour at 65°C or Ammonium Hydroxide for 8 hours at 55°C. For sensitive minor bases or dyes, Glen UnySupport may be eliminated with 50 mM Potassium Carbonate in Methanol in 17 hours at room temperature or with Tert-Butylamine/water 1:3 (v/v) for 4 hours at 60°C.Gas Phase:Methylamine gas for 30 minutes at 65°C at 30 psi.
Specifications
StorageControlled room temperature or lower, dry

Intellectual Property

This product is covered by US Patent 7,202,264 owned by Ionis Pharmaceuticals, Inc..

References

REFERENCE(S)| 1 A.P. Guzaev, and M. Manoharan, J Am Chem Soc, 2003, 125, 2380- 2381.| 2 R.K. Kumar, A.P. Guzaev, C. Rentel, and V.T. Ravikumar, Tetrahedron, 2006, 62, 4528.

Related Products

Glen UnySupport™ 500
Glen UnySupport™ 500Catalog #: 20-5040
High Load Glen UnySupport™
High Load Glen UnySupport™Catalog #: 25-5040
Glen UnySupport™ FC
Glen UnySupport™ FCCatalog #: 22-5041
Glen UnySupport™ PS
Glen UnySupport™ PSCatalog #: 26-5040
Universal Support III PS
Universal Support III PSCatalog #: 26-5010
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