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Poly(A) Polymerase catalyzes the addition of AMP from ATP to the 3′ hydroxyl of RNA. The reaction requires Mg2+ and is template independent.
Source of ProteinThe gene encoding E. coli Poly(A) Polymerase expressed from a plasmid in E. coli.
Supplied in25 mM Tris-HCl500 mM NaCl1mM MgCl20.1 mM DTT0.1 mM EDTA50% glycerolpH 8.0 @ 25°C
Supplied With:B7460 (10X Poly(A) Polymerase Reaction Buffer)N2070-10 (10 mM ATP Solution)
10X Poly(A) Polymerase Reaction Buffer (B7460):500 mM Tris-HCl2.5 M NaCl100 mM MgCl2pH 7.9 @ 25°C
Unit Definition1 unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-insoluble material in 10 minutes at 37°C.
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