Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+ as a cofactor.
Source of ProteinA recombinant E. coli strain carrying the cloned Taq DNA Ligase gene.
Supplied in10 mM Tris-HCl50 mM KCl1 mM DTT0.1 mM EDTA0.1% Tween-2050% glycerolpH 7.5 @ 25°C
Supplied With B6060 (10X Taq DNA Ligase Buffer)
10X Taq DNA Ligase Buffer (B6060)200 mM Tris-HCI250 mM KCl100 mM MgCl25 mM NAD0.1% Triton X-100pH 7.6 @ 25°C
Unit Definition1 unit is defined as the amount of Taq DNA Ligase required to join 50% of 1 µg of the 12-base cohesive ends of Lambda DNA cut with Sma I and Sal I in 50 µl 1X Taq DNA Ligase Buffer following a 10 minute incubation at 45°C.
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