T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids (1).
Source of ProteinA recombinant E. coli strain carrying the cloned T4 DNA Ligase gene.
Supplied In10 mM Tris-HCl50 mM KCl1 mM DTT0.1 mM EDTA50% glycerolpH 7.5 @ 25°C
Supplied WithB1010 (2X Rapid Ligation Buffer)B6030 (10X T4 DNA Ligase Buffer)
2X Rapid Ligation Buffer (B1010):132mM Tris-HCI20 mM MgCl22 mM DTT2 mM ATP15% PEG 6000pH 7.6 @ 25°C
10X T4 DNA Ligase Buffer (B6030):500mM Tris-HCI100 mM MgCl250 mM DTT10 mM ATPpH 7.6 @ 25°C
Unit Definition1 unit is defined as the amount of DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1X DNA Ligase Buffer following a 30 minute incubation at 23°C.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn