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Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3′ hydroxyl terminus of single or double stranded DNA molecules. The presence of 1 mM Co2+stimulates the tailing of the 3′-ends of DNA fragments. This construct is sold as an N-terminal truncation of the terminal transferase gene attached to an N-terminal fusion tag.
Source of ProteinAn E. coli strain that carries the cloned terminal transferase gene from calf thymus.
Supplied in 50 mM KPO4100 mM NaCl1.0 mM DTT0.1 mM EDTA0.1% Triton X-10050% GlycerolpH 7.3 @ 25°C
Supplied WithB0120 (10X Green Buffer)B0220 (2.5 mM CoCl2)
10X Green Buffer (B0120)200 mM Tris-Acetate500 mM Potassium Acetate100 mM Magnesium AcetatepH 7.9 @ 25°C
Unit Definition1 unit is defined as the amount of polymerase required to convert 1 nmol of dTTPs into acid insoluble material in 1 hour at 37°C.
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