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Taq DSC 2.0 DNA Polymerase is a thermally stable, processive, 5’→3′ DNA polymerase. The 94 kDa protein possesses an inherent 5’→3′ nick-translation moiety and lacks a 3’→5′ proofreading function. The DSC formulation contains a novel, nucleic-acid based hot-start additive designed to sequester the polymerase during reaction setup and during low-temperature cycling reaction phases.
Source of ProteinA recombinant E. coli strain carrying the Taq DNA polymerase gene from the thermophilic organism Thermus Aquaticus YT-1.
Supplied in20 mM Tris-HCl100 mM NaCl1.0 mM DTT0.1 mM EDTAStabilizer50% glycerolpH 7.5 @ 25°C
Supplied With B7030 (10X PCR Buffer I)
10X PCR Buffer l (B7030)100 mM Tris-HCl500 mM KCl15 mM MgCl2pH 8.3 @ 25°C
Unit Definition1 unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
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