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T7 RNA Polymerase is a DNA-dependent RNA polymerase having high specificity for the T7 promoter. After promoter initiation, it catalyzes the Mg2+ dependent synthesis of RNA from rNTPs.
Source of ProteinPurified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.
Supplied in50 mM Tris-HCl100 mM NaCl1 mM DTT0.1 mM EDTA50% glycerol0.1% Triton X-100pH 7.9 @ 25°C
Supplied WithB7180 (10X T7 RNA Polymerase Buffer)
10X T7 RNA Polymerase Buffer (B7180)400 mM Tris-HCl60 mM MgCl2100 mM DTT20 mM SpermidinepH 7.9 @ 25°C
Unit DefinitionOne unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C.
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