Manta 1.0 DNA Polymerase (exo-) is a thermophilic DNA polymerase deficient in both proofreading (3′→ 5′) and nick-translation (5′→ 3′) nuclease activities. The protein was originally characterized and its crystal structure solved by Lorena Beese (1).
Source of ProteinA recombinant E. coli strain carrying the Manta 1.0 DNA Polymerase (exo-) polymerase gene.
Supplied in10 mM Tris-HCl50 mM KCl1.0 mM DTT0.1 mM EDTA0.1% Triton X-10050% glycerolpH 7.5 @ 25°C
Supplied WithB7140 (10X PCR Buffer II)
10X PCR Buffer ll (B7140):200 mM Tris-HCl100 mM (NH4)2SO4100 mM KCl20 mM MgSO41.0% Triton X-100pH 8.8 @ 25°C
Unit Definition1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C.
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