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DNA Polymerase I is a mesophilic DNA polymerase that exhibits 5′-3′ DNA synthesis in addition to both 3′→ 5′ and 5′→ 3′ exonuclease activities. The combination of DNA synthesis and 5′→ 3′ nuclease characteristics enable nick-translation during DNA synthesis.
Source of ProteinA recombinant E. coli strain carrying the PolA gene.
Supplied in 25 mM Tris-HCl0.1 mM EDTA1.0 mM DTT50% glycerolpH 7.4 @ 25°C
Supplied WithB0110 (10X Blue Buffer)
10X Blue Buffer (B0110)500 mM NaCl100 mM Tris-HCl100 mM MgCl210 mM DTTpH 7.9 @ 25°C
Unit Definition1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37°C.
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