Jackson/BiotinSP (long spacer) AffiniPure Goat AntiRat IgG + IgM (H+L)/2.0 ml/112065044

WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.

Basedonimmunoelectrophoresisand/orELISA,theantibodyreactswithbothratIgGandIgM.Italsoreactswiththelightchainsofotherratimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.Theantibodymaycross-reactwithimmunoglobulinsfromotherspecies.

PhysicalState:Freeze-driedsolid
Storage:

Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing.
Expirationdate:oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.

Purity:Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.
Buffer:0.01MSodiumPhosphate,0.25MNaCl,pH7.6
StABIlizer:15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)
Preservative:0.05%SodiumAzide

SuggestedWorkingConcentrationorDilutionRange:
UsingFluorophoreConjugatedStreptavidin:
Histo-/Cyto-chemistry:-1:200-1:1,000
FlowCytometry:-1:200-1:1,000

UsingEnzymeConjugatedStreptavidin:
ELISA:-1:20,000-1:400,000
WesternBlots:1:20,000-1:400,000
Histo-/Cyto-chemistry:-1:500-1:5,000

Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempirically.

Biotin-SP(longspacer)

Biotin-SPisourtradenameforbiotinwitha6-atomspacerpositionedbetweenbiotinandtheproteintowhichitisconjugated.WhenBiotin-SP-conjugatedantibodiesareusedinenzymeimmunoassays,thereisanincreaseinsensitivitycomparedtobiotin-conjugatedantibodieswithoutthespacer.ThisisespeciallynotablewhenBiotin-SPconjugatedantibodiesareusedwithalkalinephosphatase-conjugatedstreptavidin.Apparently,thelongspacerextendsthebiotinmoietyawayfromtheantibodysurface,makingitmoreaccessIBLetobindingsitesonstreptavidin.Biotinylatedantibodiesrequireanadditionalreagentforvisualization.WeofferstreptavidinandMouseAnti-Biotinconjugatedtofluorophoresandenzymes.

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Jackson/BiotinSP (long spacer) AffiniPure Goat AntiRat IgG + IgM (H+L)/2.0 ml/112065044

Biotin-SP(longspacer)