Jackson/AffiniPure Fab Fragment Goat AntiRat IgG, Fcγ fragment specific/1.0 mg/112007008

FabfragmentantibodiesaregeneratedbypapaindigestionofwholeIgGantibodiestoremovetheentireFcportion,includingthehingeregion.Theseantibodiesaremonovalent,containingonlyasingleantigenbindingsite.ThemolecularweightofFabfragmentsisabout50kDa.

Basedonimmunoelectrophoresisand/orELISA,theantibodyreactswiththeFcportionofratIgGheavychainbutnotwiththeFabportionofratimmunoglobulins.NoantibodywasdetectedagainstratIgMoragainstnon-immunoglobulinserumproteins.Theantibodymaycross-reactwithimmunoglobulinsfromotherspecies.

PhysicalState:Sterile-filteredliquid
Storage:Storeat2-8°Cundersterileconditions.Prepareworkingdilutionfresheachday.
Expirationdate:oneyearfromdateofreceipt.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.

Purity:Theantibodywaspurifiedfromantiserabyacombinationofpapaindigestionandimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.FcfragmentsandwholeIgGmoleculeshavebeenremoved.
Buffer:0.01MSodiumPhosphate,0.25MNaCl,pH7.6
Preservative:None

SuggestedWorkingConcentrationorDilutionRange:

20-40μg/ml

Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeABIlity,etc.Theactualdilutionusedmustbedeterminedempirically.

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Jackson/AffiniPure Fab Fragment Goat AntiRat IgG, Fcγ fragment specific/1.0 mg/112007008