Jackson/RPhycoerythrin AffiniPure F(ab)₂ Fragment Donkey AntiRat IgG (H+L)/1.0 ml/712116150

F(ab)₂fragmentantibodiesaregeneratedbypepsindigestionofwholeIgGantibodiestoremovemostoftheFcregionwhileleavingsomeofthehingeregion.F(ab)₂fragmentshavetwoantigen-bindingFabportionslinkedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisabout110kDa.Theyareusedforspecificapplications,suchastoavoidbindingofsecondaryantibodiestolivecellswithFcreceptorsortoProteinAorProteinG.

Basedonimmunoelectrophoresisand/orELISA,theantibodyreactswithwholemoleculeratIgG.Italsoreactswiththelightchainsofotherratimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.TheantibodyhasbeentestedbyELISAand/orsolid-phaseadsorbedtoensureminimalcross-reactionwithbovine,chicken,goat,guineapig,syrianhamster,horse,human,rabbitandsheepserumproteins,butitmaycross-reactwithimmunoglobulinsfromotherspecies.

PhysicalState:Freeze-driedsolid
Storage:Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Storeat2-8°C-DONOTFREEZE.Prepareworkingdilutionfresheachday.
Expirationdate:sixmonthsfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.

Purity:Theantibodywaspurifiedfromantiserabyacombinationofpepsindigestionandimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.FcfragmentsandwholeIgGmoleculeshavebeenremoved.
Buffer:0.01MSodiumPhosphate,0.25MNaCl,pH7.6
StABIlizer:15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)
Preservative:0.05%SodiumAzide

SuggestedWorkingConcentrationorDilutionRange:
FlowCytometry:-1:50-1:200

Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempirically.

R-Phycoerythrin

Amax:488Emax:580nm

Phycoerythrin(R-PE)isamongseveralkindsoflight-harvestingphycobiliproteinsfoundinred,blue-green,andcryptomonadalgae.WeofferR-PE,theformfoundinredmacrophyticalgae(seaweed).Afterphycobiliproteinsareconjugatedtosecondaryantibodies,thereislittlefluorescencequenching,whichresultsinconjugatesofhighspecificfluorescencecomparedwithconventionalfluorophore-antibodyconjugates.R-PEcanbeexcitedbylightoverawiderangeofthevisIBLespectrumishighlywatersoluble,hasarelativelylowisoelectricpoint,andlackspotentiallystickycarbohydrates.

ItshouldbenotedthattherelativelyhighmolecularweightofR-PEmayprecludeitsuseinproceduresrequiringgoodpenetrationintocellsandtissues.Itispredominantlyintendedforsurfacelabelingofcellsforflowcytometry.

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Jackson/RPhycoerythrin AffiniPure F(ab)₂ Fragment Donkey AntiRat IgG (H+L)/1.0 ml/712116150

R-Phycoerythrin