Jackson/Alkaline Phosphatase AffiniPure Goat AntiHuman IgG + IgM (H+L)/1.0 ml/109055127

WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.

Basedonimmunoelectrophoresisand/orELISA,theantibodyreactswithbothhumanIgGandIgM.Italsoreactswiththelightchainsofotherhumanimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.TheantibodyhasbeentestedbyELISAand/orsolid-phaseadsorbedtoensureminimalcross-reactionwithbovineserumproteins,butitmaycross-reactwithimmunoglobulinsfromotherspecies.

PhysicalState:Freeze-driedsolid
Storage:Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.
Expirationdate:oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.

Purity:Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.
Buffer:0.01MTris-HCl,0.25MNaCl,pH8.0
StABIlizer:15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)
Preservative:0.05%SodiumAzide

SuggestedWorkingConcentrationorDilutionRange:
ELISA:-1:5,000-1:50,000
WesternBlot:-1:5,000-1:50,000

Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempirically.

AlkalinePhosphatase

Alkalinephosphatase(fromcalfintestine)conjugatesarepreparedbyamodifiedmethodofAvremeasetal.,Scand.J.Immunol.1978.8(Supple.7),7.Resultingconjugatescontainheterogeneous,highmolecularweightcomplexes.Theyaresensitivereagentsforsolid-phaseimmunoassayssuchasELISAandWesternblotting.Althoughalkalinephosphataseconjugatesaresometimesusedforimmunohistochemistry,penetrationintowholemounttissuesmaybelimitedbytheirlargesizes.

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Jackson/Alkaline Phosphatase AffiniPure Goat AntiHuman IgG + IgM (H+L)/1.0 ml/109055127

AlkalinePhosphatase