Jackson/Brilliant Violet 480™ AffiniPure Donkey AntiChicken IgY (IgG) (H+L)/0.25 ml/703685155

WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.

Basedonimmunoelectrophoresisand/orELISA,theantibodyreactswithwholemoleculechickenIgY.Italsoreactswiththelightchainsofotherchickenimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.TheantibodyhasbeentestedbyELISAand/orsolid-phaseadsorbedtoensureminimalcross-reactionwithbovine,goat,guineapig,syrianhamster,horse,human,mouse,rabbit,ratandsheepserumproteins,butitmaycross-reactwithimmunoglobulinsfromotherspecies.

PhysicalState:Sterile-filteredliquid
Storage:

Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing.
Expirationdate:oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.
Note:Storestainedspecimensat4°Ctopreservefluorescence.

Purity:Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.
Buffer:0.01MSodiumPhosphate,0.15MNaCl,0.005%Tween20,pH7.2
Stabilizer:15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)
Preservative:0.05%SodiumAzide

SuggestedWorkingConcentrationorDilutionRange:

1:50-1:200formostapplications

Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempirically.

BrilliantViolet480™

Amax:436Emax:478nm

BDBrilliant™Violet480(BV480)hasanExcitationpeakat436nmwithmaximalemissionat478nm.BrilliantVioletpolymerchainscanbeconsideredasacollectionofopticalsegments,eachwiththeabilitytoabsorblightandemitfluorescencesignal.Thisresultsinmaterialsthathaveabrightfluorescencesignalforbetterresolutionandsensitivity.

Ifnuclearcounterstainingisdesired,4-colorantibodystainingispossibleusingBrilliantViolet™421,BrilliantViolet™480,AlexaFluor®488andRhodamineRed™-X.Ifnuclearcounterstainingisdesired,4-colorantibodystainingispossibleusingBrilliantViolet™421,BrilliantViolet™480,AlexaFluor®488andRhodamineRed™-X.SwitchingthenuclearstainfromDAPI(emissionintheblueregion)toDRAQ5™(whichhasredemission)freestheviolet-blueregionofthespectrumtoaccommodatethetwoBrilliantVioletdyes.DRAQ5’sexcitationandemissionprofilesoverlapthoseofAlexaFluor647.

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Jackson/Brilliant Violet 480™ AffiniPure Donkey AntiChicken IgY (IgG) (H+L)/0.25 ml/703685155

BrilliantViolet480™