Jackson/Fluorescein (FITC) AffiniPure Goat AntiRabbit IgG (H+L)/1.5 mg/111095144

WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.

Basedonimmunoelectrophoresisand/orELISA,theantibodyreactswithwholemoleculerabbitIgG.Italsoreactswiththelightchainsofotherrabbitimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.TheantibodyhasbeentestedbyELISAand/orsolid-phaseadsorbedtoensureminimalcross-reactionwithhuman,mouseandratserumproteins,butitmaycross-reactwithimmunoglobulinsfromotherspecies.

PhysicalState:Freeze-driedsolid
Storage:

Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing.
Expirationdate:oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.

Purity:Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.
Buffer:0.01MSodiumPhosphate,0.25MNaCl,pH7.6
Stabilizer:15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)
Preservative:0.05%SodiumAzide

SuggestedWorkingConcentrationorDilutionRange:
Histo-/Cyto-Chemistry:-1:50-1:200
FlowCytometry:-1:50-1:200

Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempirically.

Fluorescein(FITC)

Amax:492Emax:520nm

FITC(Fluoresceinisothiocyanate)istheformoffluoresceinusedforconjugationtoallofourantibodiesandpurifiedproteins,withtheexceptionofstreptavidin.Fluoresceinconjugatesabsorblightmaximallyat492nmandfluorescemaximallyat520nm.Althoughlessbrightthanothergreen-fluorescingdyes,FITCisstillawidelyusedfluorophoreduetoitslonghistory.Themajordisadvantageoffluoresceinisitsrapidphotobleaching(fading),whichcanbemitigatedbytheuseofananti-fadingagentinthemountingmedium.AbetterchoiceformanyapplicationsinvolvingFITCisAlexaFluor®488becauseitisbrighterandmorephotostable.

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Jackson/Fluorescein (FITC) AffiniPure Goat AntiRabbit IgG (H+L)/1.5 mg/111095144

Fluorescein(FITC)