Jackson/RPhycoerythrin ChromPure Donkey IgG, F(ab)2 fragment/1.0 ml/017110006

ChromPureisourtradenameforhighlypurifiedproteinsfromtheserumofnon-immunizedanimals.

PhysicalState:Freeze-driedsolid
Storage:Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Storeat2-8°C-DONOTFREEZE.Prepareworkingdilutionfresheachday.
Expirationdate:sixmonthsfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.

Purity:Basedonimmunoelectrophoresisatanantigenconcentrationof20mg/ml,thepatternofprecipitationagainstrabbitanti-donkeywholeserumisthesameasthatagainstgoatanti-horseIgG,F(ab)2fragmentspecific.Noprecipitinlinewasdetectedagainstrabbitanti-donkeyIgG,Fcfragmentspecific.
Buffer:0.01MSodiumPhosphate,0.25MNaCl,pH7.6
Stabilizer:15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)
Preservative:0.05%SodiumAzide

R-Phycoerythrin

Amax:488Emax:580nm

Phycoerythrin(R-PE)isamongseveralkindsoflight-harvestingphycobiliproteinsfoundinred,blue-green,andcryptomonadalgae.WeofferR-PE,theformfoundinredmacrophyticalgae(seaweed).Afterphycobiliproteinsareconjugatedtosecondaryantibodies,thereislittlefluorescencequenching,whichresultsinconjugatesofhighspecificfluorescencecomparedwithconventionalfluorophore-antibodyconjugates.R-PEcanbeexcitedbylightoverawiderangeofthevisiblespectrumishighlywatersoluble,hasarelativelylowisoelectricpoint,andlackspotentiallystickycarbohydrates.

ItshouldbenotedthattherelativelyhighmolecularweightofR-PEmayprecludeitsuseinproceduresrequiringgoodpenetrationintocellsandtissues.Itispredominantlyintendedforsurfacelabelingofcellsforflowcytometry.

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Jackson/RPhycoerythrin ChromPure Donkey IgG, F(ab)2 fragment/1.0 ml/017110006

R-Phycoerythrin