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Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing.
Expirationdate:oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.
DyLight405-conjugatedsecondaryantibodiesareexcitedmaximallyatabout400nmandfluorescewithapeakatabout421nm.Theyareverybrightandphotostable,buttheiroptimaluseislimitedtomicroscopesorflowcytometersequippedwitha405nmlaseranda420nmemissionfilter.Undertheseconditions,itispossibletoperformeffective4-colorimagingwithgoodcolorseparation,goodphotostability,andhighsensitivity.ThecombinationofDyLight405,AlexaFluor®488,RhodamineRed-X,andAlexaFluor®647providesformaximumcolorseparation.Another4-colordyecombination,whichmaybeequallyeffectivebuthasslightlylesscolorseparation,isDyLight405,AlexaFluor®488,Cy3,andAlexaFluor®647.DyLight405conjugatesareanexcellentchoiceforblue-fluorescingsecondaryantibodiesinmulti-colorlabelingprotocols.
