Jackson/PerCP ChromPure Donkey IgG, F(ab)2 fragment/0.5 mg/017120006

ChromPureisourtradenameforhighlypurifiedproteinsfromtheserumofnon-immunizedanimals.

PhysicalState:Freeze-driedsolid
Storage:Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Storeat2-8°C-DONOTFREEZE.Prepareworkingdilutionfresheachday.
Expirationdate:sixmonthsfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.

Purity:Basedonimmunoelectrophoresisatanantigenconcentrationof20mg/ml,thepatternofprecipitationagainstrabbitanti-donkeywholeserumisthesameasthatagainstgoatanti-horseIgG,F(ab)2fragmentspecific.Noprecipitinlinewasdetectedagainstrabbitanti-donkeyIgG,Fcfragmentspecific.
Buffer:0.01MSodiumPhosphate,0.25MNaCl,pH7.6
Stabilizer:15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)
Preservative:0.05%SodiumAzide

PerCP

Amax:488Emax:675nm

PerCPisafluorescentperidinin-chlorophyll-proteincomplexisolatedfromdinoflagellates.WeoffertheformfoundinDinophyceaesp.withamolecularweightofabout35.5kDa.Ithasabroadspectrumofexcitationwithamainpeakat482nm,andalongStokesshifttoanemissionpeakat677nm.

PerCP,AlexaFluor®488(orFITC),andR-PEareexcitedat488nmwithanargonlaser,andthuscanbeusedforone-,two-,andthree-coloranalyseswithsingle-laserflowcytometers.APCandAlexaFluor®647areexcitedat633nmtogiveafourthcolorwithdual-laserflowcytometers.

PerCPisalsohighlywatersoluble,hasarelativelylowisoelectricpoint,andlackspotentiallystickycarbohydrates.

ItshouldbenotedthattherelativelyhighmolecularweightofPerCPmayprecludeitsuseinproceduresrequiringgoodpenetrationintocellsandtissues.Itispredominantlyintendedforsurfacelabelingofcellsforflowcytometry.

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Jackson/PerCP ChromPure Donkey IgG, F(ab)2 fragment/0.5 mg/017120006

PerCP