企业QQ
官方微信
FabfragmentantibodiesaregeneratedbypapaindigestionofwholeIgGantibodiestoremovetheentireFcportion,includingthehingeregion.Theseantibodiesaremonovalent,containingonlyasingleantigenbindingsite.ThemolecularweightofFabfragmentsisabout50kDa.
Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing.
Expirationdate:oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.
AlexaFluor®488-conjugatedantibodiesabsorblightmaximallyat493nmandfluorescewithapeakaround519nm.InaqueousmountingmediatheyarebrighterthanFITC,Cy2,andDyLight488.AlexaFluor®488conjugatesarerecommendedformaximumsensitivityforallimmunofluorescenceproceduresrequiringagreen-fluorescingdye,exceptforprotocolsthatincludemountinginplasticmountingmedia.
