Jackson/Alkaline Phosphatase AffiniPure Goat AntiMouse IgG, Fcγ Subclass 2c Specific/0.5 ml/115055

WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.

Basedonantigen-bindingassayand/orELISA,theantibodyreactswiththeFcportionofmouseIgG2cbutnotwithothermouseIgGsubclasses,mouseIgM,ortheFabportionofmouseimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.TheantibodyhasbeentestedbyELISAand/orsolid-phaseadsorbedtoensureminimalcross-reactionwithhuman,bovineandrabbitserumproteins,butitmaycross-reactwithimmunoglobulinsfromotherspecies.

PhysicalState:Freeze-driedsolid
Storage:Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.
Expirationdate:oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.

Purity:Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.
Buffer:0.01MTris-HCl,0.25MNaCl,pH8.0
Stabilizer:15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)
Preservative:0.05%SodiumAzide

SuggestedWorkingConcentrationorDilutionRange:
ELISA:-1:5,000-1:50,000
WesternBlot:-1:5,000-1:50,000

Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempirically.

AlkalinePhosphatase

Alkalinephosphatase(fromcalfintestine)conjugatesarepreparedbyamodifiedmethodofAvremeasetal.,Scand.J.Immunol.1978.8(Supple.7),7.Resultingconjugatescontainheterogeneous,highmolecularweightcomplexes.Theyaresensitivereagentsforsolid-phaseimmunoassayssuchasELISAandWesternblotting.Althoughalkalinephosphataseconjugatesaresometimesusedforimmunohistochemistry,penetrationintowholemounttissuesmaybelimitedbytheirlargesizes.

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

Jackson/Alkaline Phosphatase AffiniPure Goat AntiMouse IgG, Fcγ Subclass 2c Specific/0.5 ml/115055

AlkalinePhosphatase