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Jackson/12 nm Colloidal Gold AffiniPure Donkey AntiMouse IgG (H+L) (EM Grade)/0.3 ml/715205150188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Jackson/12 nm Colloidal Gold AffiniPure Donkey AntiMouse IgG (H+L) (EM Grade)/0.3 ml/715205150

WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.

Basedonimmunoelectrophoresisand/orELISA,theantibodyreactswithwholemoleculemouseIgG.Italsoreactswiththelightchainsofothermouseimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.TheantibodyhasbeentestedbyELISAand/orsolid-phaseadsorbedtoensureminimalcross-reactionwithbovine,chicken,goat,guineapig,syrianhamster,horse,human,rabbitandsheepserumproteins,butitmaycross-reactwithimmunoglobulinsfromotherspecies.
PhysicalState:Sterile-filteredliquid
Storage:

Aliquotandfreezeundilutedproductat-20°Corbelow.Avoidrepeatedfreezingandthawing.Prepareworkingdilutionfresheachday.
Expirationdate:sixmonthsfromdateofreceipt.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.


Purity:Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.
Buffer:0.01MSodiumBorate-SodiumPhosphate,0.15MNaCl,pH8.5
Stabilizer:15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)
Preservative:0.05%SodiumAzide

SuggestedWorkingConcentrationorDilutionRange:
Histo-/Cyto-Chemistry:-1:20-1:40

Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempirically.

12nmColloidalGold



Colloidalgoldreagentsfortransmissionandscanningelectronmicroscopy(EMGrade)aredistinguishedfromothercommercialpreparationsbycarefulseparationofmonomericparticlesfromsmallaggregatesusingultracentrifugationindensitygradients.Theresultingmonomericcolloidalgold-proteincomplexesaresuspendedinsterile-filteredbuffercontainingstabilizersandapreservative.AllparticlesizesintheEMGradecategorymayalsobeusedforlightmicroscopyandimmunoblottingbythosewhoprefertouseparticleslargerthan4nm.


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