Jackson公司112-165-003/Cy3-AffiniPureGoatAnti-RatIgG现货价格低

2.0mg

WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.
Basedonimmunoelectrophoresisand/orELISA,theantibodyreactswithwholemoleculeratIgG.Italsoreactswiththelightchainsofotherratimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.Theantibodymaycross-reactwithimmunoglobulinsfromotherspecies.
PhysicalState: Freeze-driedsolid
Storage:
Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing.
Expirationdate: oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.
Purity: Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.
Buffer: 0.01MSodiumPhosphate,0.25MNaCl,pH7.6
Preservative: 0.05%SodiumAzide
SuggestedWorkingConcentrationorDilutionRange:
1:100-1:800formostapplications
Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeABIlity,etc.Theactualdilutionusedmustbedeterminedempirically.
美国JacksonImmunoResearchLaboratories,Inc.专业致力于亲合纯化的第二抗体和纯化的免疫球蛋白的生产和偶联。其产品深受广大研究人员的认可,销售范围遍布全球,领域包括植物,动物和生物医学研究。公司成立于1982年,公司创业者具有深厚的专业背景:细胞生物学,蛋白化学,微生物学,免疫学。公司自创立初,就将为其他研究人员提供最新最全的二抗作为公司的持续发展目标。