PNA oligo can be used as a sequence specific PCR blocker because PNA probes have strong binding affinity and specificity to its target DNA and not being recognized by DNA polymerase as primer. This method is also called PNA Clamping. PCR Blocker or PNA Clamping is an efficient way to correct amplification bias and sequencing errors.
This is one way to achieve SNP specific genotyping of the gene of interest, such as mutant screening.
One of the examples of this approach is globin reduction PNA. Using a mixture of PNA that is specific for human blood gamma globin RNA, the PNA suppresses the amplification of the globin RNA during cDNA synthesis for microarray application.
The other popular application of PNA as PCR blocker is mRNA and pPNA. These two PNA oligos are made against chloroplast and mitochondrial 16S sequences from diverse plant species. They are proven to be a useful in megagenomics.
PNA oligo can be custom made for each application. For more detail, please refer Custom PNA Oligos.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn