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ProChimia/HS-(CH2)m-COO-NHS/FT 003-m11-0.2/200 mg188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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ProChimia/HS-(CH2)m-COO-NHS/FT 003-m11-0.2/200 mg

Description

Harbors molecules onto a SAM-covered surface though an amide bond forming between the NHS ester and the molecule's free amino group. This NHS-ester coupling reaction is extremely easy to carry out, and proceeds in excellent yield. In a typical procedure, a gold slide (~1 cm2) covered with NHS-terminated SAM is immersed for 3 hrs in 0.5 mL of a methylene chloride (chloroform, THF, dry DMF and DMSO, absolute ethanol are also allowed) solution of a molecule bearing free amino group and 1.5 equivalents of NEt3. When the reaction is complete, the substrate should be rinsed with pure solvent and dried under a stream of nitrogen.
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