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FuGENE®HDTransfectionReagentisanext-generationtransfectionreagent,freeofanimal-derivedcomponents.Itisdesignedtotransfectawiderangeofeukaryoticcellsincludinginsectcellsandmanycelllinesnottransfectedwellbyotherreagents(e.g., MCF-7,RAW264.7,PC-3,HeLa,MA-10,HepG2,SH-SY5Y,A7r5,STO,SCC-61,STSAR-90,SQ20B,T98).
Note:Forthemostup-to-datelistofcelltypesthathavebeensuccessfullytransfectedwithFuGENE®HDTransfectionReagent,visitourTransfectionSpecialInterestSiteatwww.powerful-transfection.comandviewourTransfectionReferenceDatabase.Thissitealsocontainsmuchmoreinformationabouttheuseofthisreagent.
Forlifescienceresearchonly.
ProductDescription
Composition:uniqueandproprietaryblendofmanycomponents, in80%ethanol.
Storage: stableat+2°Cto+8°C.
StABIlity: 2years,startingfromdateofmanufacture.
Contents:
Solutionin80%ethanol,filter-sterilized(0.1µmfilter),packagedintransparentglassvials.
Quality:
Lot-specificanalysis:
EachlotofFuGENE®HDTransfectionReagentiscarefullychecked,using ISODIN9001-certified procedures,toassureproductisperformingaccordingtospecificationsandisconsistentfromlottolot.
Functionalanalysis:
Cos7cellsaretransfectedin96wellplateswithareportergenevectorDNA,using0.3µlFuGENE®HDand0.1µgDNAor0.25µlFuGENE®HDand0.1µgDNA.Reportergeneactivityismonitoredviaacolorimetrictestagainstareferencelot.Activitymustbe≥ 75%comparedtoareferencelotinatleastoneofthetestedamounts,listedabove.
Cytotoxicityanalysis:
CellgrowthisassessedusingtheCellProliferationReagentWST-1assay whichisanindirectmeasureofcytotoxicity.Theremainderofcellsfromfunctionalanalysisistestedforviability.
目录编号 名称 包装 单价 04709691001 FuGENE®HD TransfectionReagent Roche公司产品 0.4ml（多达120次转染） 1907元 04709705001 1.0ml（多达300次转染） 3881元 04709713001 5x1ml（多达1,500次转染） 17396元 03036421001 X-tremeGENEQ2 TransfectionReagent 1.8ml（多达450次转染 询价 03045595001 0.4ml（多达100次转染） 询价 04476093001 X-tremeGENEsiRNA 1ml（24孔板中多达400次） 2411元 04476115001 5x1ml（中多达2,000） 10256元 11811177001 DOTAP 脂质体转染试剂 0.4ml 1386元 11202375001 2ml（5x0.4ml，2mg） 3410元 11811169001 DOSPER 脂质体转染试剂 0.4ml 询价 11781995001 2ml（5x0.4ml，2mg） 询价 上海市徐汇区南丹东路18号12楼A座 北京朝阳区大屯路2号科华商务大厦613室 021-34615995 021-34615975 010-64847623 010-64855926
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