FuGENE® HDTransfectionReagentisanext-generationtransfectionreagent,freeofanimal-derivedcomponents.Itisdesignedtotransfectawiderangeofeukaryoticcellsincludinginsectcellsandmanycelllinesnottransfectedwellbyotherreagents( Note: Forthemostup-to-datelistofcelltypesthathavebeensuccessfullytransfectedwith FuGENE® HDTransfectionReagent, visitour TransfectionSpecialInterestSite at andviewourTransfectionReferenceDatabase.Thissitealsocontainsmuchmoreinformationabouttheuseofthisreagent. Forlifescienceresearchonly. Composition: uniqueandproprietaryblendofmanycomponents, in80%ethanol. Storage: stableat+2°Cto+8°C. StABIlity: 2years,startingfromdateofmanufacture. Functionalanalysis:
EachlotofFuGENE®HDTransfectionReagentiscarefullychecked,using ISODIN9001-certified procedures,toassureproductisperformingaccordingtospecificationsandisconsistentfromlottolot.
Cos7cellsaretransfectedin96wellplateswithareportergenevectorDNA,using0.3µlFuGENE® HDand0.1µgDNAor0.25µlFuGENE® HDand0.1µgDNA.Reportergeneactivityismonitoredviaacolorimetrictestagainstareferencelot.Activitymustbe≥ 75%comparedtoareferencelotinatleastoneofthetestedamounts,listedabove.
Cytotoxicityanalysis:
Cellgrowthisassessedusingthe CellProliferationReagentWST-1assay which isanindirectmeasureofcytotoxicity.Theremainderofcellsfromfunctionalanalysisistestedforviability.xy-E12443 【中文名称】:大鼠CXC趋化因子配体16(CXCL16)ELISA试剂盒 【英文名称】:RatsCXCchemokineligand16(CXCL16)ELISAKit 规格:96T/48T
xy-E12444 【中文名称】:大鼠高敏三碘甲状腺原氨酸(u-T3)ELISA试剂盒 【英文名称】:Threehighsensitivityinratstriiodothyronine(u-T3)ELISAkit 规格:96T/48T
xy-E12445 【中文名称】:大鼠BH3结构域凋亡诱导蛋白(Bid)ELISA试剂盒 【英文名称】:BH3domainofratapoptosis-inducingprotein(Bid)ELISAkit 规格:96T/48T
xy-E12446 【中文名称】:大鼠胱天蛋白酶9(Casp-9)ELISA试剂盒 【英文名称】:Ratscaspase9(Casp-9)ELISAKit 规格:96T/48T
xy-E12447 【中文名称】:大鼠阿立新A(OrexinA)ELISA试剂盒 【英文名称】:RatorexinA(OrexinA)ELISAkit 规格:96T/48T
xy-E12448 【中文名称】:大鼠胱天蛋白酶3(Casp-3)ELISA试剂盒 【英文名称】:Ratscaspase3(Casp-3)ELISAKit 规格:96T/48T
xy-E12449 【中文名称】:大鼠B细胞淋巴瘤因子2(Bcl-2)ELISA试剂盒 【英文名称】:RatB-celllymphomafactor2(Bcl-2)ELISAKit 规格:96T/48T
xy-E12450 【中文名称】:大鼠CXC趋化因子受体3(CXCR3)ELISA试剂盒 【英文名称】:RatCXCchemokinereceptor3(CXCR3)ELISAkit 规格:96T/48T
xy-E12451 【中文名称】:大鼠中性粒细胞弹性蛋白酶(NE)ELISA试剂盒 【英文名称】:Ratneutrophilelastase(NE)ELISAKit 规格:96T/48T
xy-E12452 【中文名称】:大鼠肿瘤特异生长因子/肿瘤相关因子(TGSF)ELISA试剂盒 【英文名称】:Rattumorspecificgrowthfactor/tumor-associatedfactor(TGSF)ELISAkit 规格:96T/48T
xy-E12453 【中文名称】:大鼠羟脯氨酸(Hyp)ELISA试剂盒 【英文名称】:Ratshydroxyproline(Hyp)ELISAKit 规格:96T/48T
xy-E12454 【中文名称】:大鼠骨保护素配体(OPGL)ELISA试剂盒 【英文名称】:Ratosteoprotegerinligand(OPGL)ELISAkit 规格:96T/48T
xy-E12455 【中文名称】:大鼠乳铁传递蛋白/乳铁蛋白(LF/LTF)ELISA试剂盒 【英文名称】:Ratslactoferrin/lactoferrin(LF/LTF)ELISAKit 规格:96T/48T