Nature Biotech：肿瘤异质性图谱

HomeAntibodiesOrder NowServicesContactSupportMoreUse tab to navigate through the menu items.Order 0CARTTransmission Electron Microscopy:TEM cryo-immunnohistochemistry of afrozen thin section of human lung using anti HT1-56 to visulize the apical surface of type 1 cells (both upper panels) andHT2-280 localized tothe apical surface of type 2 cells (bottom panel).Site TitleDifferential Cell Staining: Mixed alveolar epithelial cells isolated from an injured human lung specimen, cultured for 5 days on fibronectin coated plastic promoting the type 1 cell phenotype, and differentially stained with anti HT1-56 (green) and anti HT2-280 (red). Type 1 cells are stained green, attenuated type 2 cells arered, and transitional dual staining cells are yellow.Immunohistochemistry:Human type 1 cells isolated using anti HT1-56 and cultured on fibronectin coated plastic for 5days forming a contact inhibited cell monolayer (in red), and rat type 1 cells isolated using anti RT1-40 and similarly cultured for 6 days (in green).Our antibodies, specific within the human lung to alveolar epithelial type 1or type 2cells, are validated and effective for: - Immunohistochemistry - Western Blotting - Cell Isolation by FACS - Semi-Quantitative Dot Blots - Magnetic Cell Sorting - TEM Cryo-Immunohistochemistry Human lung alveolar type 2cell labeled withantibody to HT2-280 with bound gold nanospheres. 0.1micron: Providing validated antibodies for innovative research.Terrace Biotech Examples of Possible Applications of Anti HT1-56and Anti HT2-280to Advance Your Research.Immunohistochemistry: Anti HT1-56and anti HT2-280 used in conjunction to differentially illuminate alveolar type1 cells (red) and type 2 cells (green) in a paraformaldehyde fixed thinsection of a human lung specimen.Cell Isolation by FACS: Immunofluorescence image of FACS isolated human type 1and type 2cells showing high purity for each differentially stained population. (A) HT1-56 positive cells (red), DAPI stained nuclei (blue). (C) HT2-280 positive cells (green). (B D) Accompanying phase contrast images for A and C. A.Western Blotting: Western Blots of whole lung homgenate run on a (A)7% SDS-PAGEgel run under reducing conditions,transfered onto nitrocellulose, and probed using anti HT2-280, or (B) homogenate run on a 10% SDS-PAGEgel run under non-reducing conditions, and probed using anti HT1-56.Semi-Quantitative Dot Blot: Anti HT1-56 used in a semi-quantitative dot blot to detectHT1-56 inhuman lung homogenate and lung aspirates from three different diseased lungs.ABOUT TERRACE BIOTECHSign up for the Terrace Biotech Newsletter and receive newproduct informationandapplication notes.© 2023 BY EZ ELECTRONICS. PROUDLY CREATED WITH WIX.COMTerrace Biotech s expertise arose from the Pulmonary Research Group at UCSF Medical Center that helped characterizepulmonary surfactant and then developed a synthetic form of it that isused to treat premature infants with respiratory distress syndrome. Withover twenty-five years of handson researchexperience inlung cell biology, Terrace Biotech applies itsexpertise to further innovation in medical research and improve human health.

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近日来自斯坦福大学的研究人员开发了一种新型的单细胞PCR微流体技术，并利用这一技术对数百个结肠癌细胞进行了单细胞基因表达分析，由此获得了人类结肠癌异质性图谱。相关研究成果发表在《自然生物技术》（Nature Biotechnology）杂志上。

肿瘤异质性是恶性肿瘤重要特征之一。肿瘤异质性可以表现在肿瘤分化水平及肿瘤功能水平上，出现异质性的抗原表达或出现不同生物特性细胞亚群。这种肿瘤异质性特性往往给肿瘤的研究和治疗带来极大的困难。

“在过去的数年里，我们一直致力于开发出一种单细胞基因组分析技术，”文章的资深作者、斯坦福大学工程学院及生物工程和应用物理系教授Stephen Quake说：“在新研究中我们利用开发的新技术获得了可喜的具有重要生物学意义的成果。”

研究人员利用这项新技术逐个地对细胞中的基因表达情况进行了分析，从中鉴别出了47种差异性表达的基因，并基于这些结果对结肠癌组织的细胞进行了亚型分类。

“在一次实验中，我们获得了特定组织细胞组成的‘快照’，鉴别出了不同的细胞亚型，并找到了识别这些细胞的新型标记物，”文章的第一作者Tomer Kalisky说。

新研究的重要发现之一就是揭示了肿瘤异质性产生的根源。长久以来科学家们认为肿瘤是在演进的过程中失去了基因组稳定，发生随机变化而生成了不同生物特性细胞亚群。新研究发现结肠癌细胞形成遵循了正常结肠细胞的分化过程。打个比喻，就如同漫画肖像，绘制的是同一张脸谱，只是某些特征被充分夸大了。癌细胞形成机制与正常细胞完全相同，只是小部分的细胞类型发生了不正常的扩大或减少。

“我们观察到结肠癌组织显示了与正常结肠上皮相似的谱系多样性，其中包括了不成熟组细胞样细胞和成熟的特化细胞，”Dalerba说。

此外，分析结果还显示在肿瘤组织中基因表达上越是接近不成熟细胞的细胞越是呈现恶性特征。基于这些结果，研究人员开发出了一种两基因分类系统，并证实相比于常规的病理学分级，这一简单的分析系统能够更准确地确定疾病的预后。

研究人员期望在未来能将这些技术广泛地应用到癌症的诊疗和预后分析中。

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Lung alveolar cell specific antibody, type 1 cell, type 2 cell, HT2-280, HT1-56