美国MTIglobalstem总代理GST2161/现货

　　产品介绍：

产品名称：DNA-In®CRISPR Transfection Reagent

产品描述：

NEW DNA-In® CRISPR Transfection Reagent

Specifically formulated for CRISPR/Cas9 transfection reagent

DNA-In® CRISPR transfection reagent is optimized for large plasmid delivery of CRISPR/Cas9 vectors into a range of cell types. This reagent is especially well-suited for hard-to-transfect primary cells. Below, a Cas9-GFP plasmid was delivered into primary fibroblasts, muscles cells, keratinocytes and others primary cells and commonly used cell types with high efficiency and low toxicity.

Figure 1. DNA-In® CRISPR delivers Cas9-GFP expression vector with maximum efficiency - Human primary fibroblasts, humanprimary keratinocytes, primary human endothelial cells (HuVEC), human skeletal muscle cells, HeLa cells and C2C12 mouse myoblasts were plated in 24-well plates in complete medium without antibiotics at the optimal plating density for each cell type to yield 50-70% confluency at the time of transfection. Cells were transfected with the pSpCas9(BB) -2A-GFP pasmid (9.3kb )(source: Addgene), using DNA-In® CRISPR reagent and incubated overnight at 37°C. Cells were then fixed and stained with anti-GFP and anti-Cas9 antibodies to better observe the transfection efficiency.

Learn about High Efficiency CRISPR-Cas9 Delivery in Stem Cells

Highly efficient Cas9 delivery in primary cells

Human dermal primary fibroblasts (above) were plated to give ~70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating. Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-In® CRISPR transfected cells (left image) show significantly higher Cas9 transfection efficiency vs cells transfected with competitor reagents (L2K, L3K).

Human primary keratinocytes (above) were plated to give a 50-70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating. Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-In® CRISPR transfected cells (left image) show significantly higher Cas9 transfection efficiency vs cells transfected with competitor reagents (L2K , L3K).

C2C12 mouse myoblasts (above) were plated to give a 50-70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating. Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-In® CRISPR transfected cells (left image) show significantly higher Cas9 transfection efficiency vs cells transfected with competitor reagents (L2K, L3K).

Human skeletal muscle cells (above) were plated to give a 50-70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating. Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-In® CRISPR transfected cells (left image) show significantly higher Cas9 transfection efficiency vs cells transfected with competitor reagents (L2K, L3K).

　　公司介绍

mti-globalstem，是生命科学领域前沿工具和试剂的供应商。mti-globalstem提供优势产品在转染试剂、神经科学、干细胞生物学。他们填补了原代和ipsc获得神经细胞、干细胞和饲养层细胞的空白，优化了生长培养基和补充物质，提供了一个贵族试剂的创新平台——最大效率的递送核酸到干细胞和神经细胞。

MTI-GlobalStem公司成立于2006年，最初名为GlobalStem，在2010年被Molecular Transfer公司(MTI)收购，总部位于美国马里兰州盖瑟斯堡，致力于开发和推广细胞转染、神经生物学以及干细胞研究所用的工具和试剂。此外，这家公司还提供干细胞、分化神经元和大规模的细胞培养制造服务。

蚂蚁淘生物是美国MTI-globalstem公司,GST-2161-DNA-In®CRISPR Transfection Reagent/现货/代理商