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MaterialsDEPC H2O DEPC 0.1% (v/v)q.s. de-ioinized H2O37ºC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide solutions should also be DEPC treated. Tris has a reactive amine and can''t be DEPC treated)10x Formaldehyde gel loading buffer: 50% glycerol10 mM EDTA, pH80.25% bromphenol blue0.25% xylene cyanol10x MOPS buffer (1L)41.8 g MOPS (0.2M) in DEPC H2O, pH720 mL 1 M NaOAC (DEPC treated) (final 20 mM)20 mL 0.5M EDTA, pH8 (DEPC treated) (final 10 mM)q.s. 1L DEPC H2OSterile filter, store at 4ºC in dark. (Don''t use if dark yellow)1x Reaction buffer (per sample)2 µL 10x MOPS buffer (final 1x)4 µL formaldehyde (final 20%)10 µL formamide (final 50%)2 µL 0.2 mg/mL ethidium bromide, DEPC treated (final 10 µg/mL)1.5% Agarose 2.2M formaldehyde gel 1.5 g agarose72 mL H2ODissolve in microwave and then cool to 55ºCIn a fume hood add:10 mL 10x MOPS buffer18 mL de-ionized formaldehydeCast gel and wrap in Saran wrap until ready to use.Note: Northern blots require formadehyde in the gel. For simple inspection, however, it is fine to use regular DNA-style TBE agarose gels. In either case, the RNA should initially be denatured (steps 2-3) and RNAse free reagents should be used. Procedure