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Bpsbioscience/PDE7A Assay Kit/60373/384 reactions

Background:
Phosphodiesterases(PDEs)playanimportantroleinthedynamicregulationofcAMPandcAMPsignaling.PDE7Aiswidelyexpressedinvarioustissuesincludingskeletalmuscle,Tlymphocytes,brainandpancreasandplaysandanimportantroleintheregulationofosteoblasticdifferentiation.
Description:
ThePDE7AAssayKitisdesignedforidentificationofPDE7Ainhibitorsusingfluorescencepolarization.TheassayisbasedonthebindingofafluorescentnucleotidemonophosphategeneratedbyPDE7Atothebindingagent.PDE7Acatalyzesthehydrolysisofthephosphodiesterbondindye-labeledcyclicadenosinemonophosphate(cAMP).Nanoparticlebeadsselectivelybindthephosphategroupinthenucleotideproduct.ThisincreasesthesizeofthenucleotiderelativetounreactedcAMP.Sincethedegreeofpolarizationofafluorophoreisinverselyrelatedtoitsmolecularrotation,dyesattachedtotheslowly-rotatingnucleotide-beadcomplexeswillnothavetimetoreorientandthereforewillemithighlypolarizedlight.Conversely,dyesattachedtotherapidly-rotatingcyclicmonophosphateswillobtainrandomorientationsandemitlightwithlowpolarization.ThekeytothePDE7AAssayKitisthespecificbindingagent.Usingthiskit,onlytwosimplestepsonamicrotiterplatearerequiredforPDE7Areactions.First,thefluorescentlylabeledcAMPisincubatedwithasamplecontainingPDE7Afor1hour.Second,abindingagentisaddedtothereactionmixtoproduceachangeinfluorescentpolarizationthatcanthenbemeasuredusingafluorescencereader.
Synonym(s):
PDE7A,phosphodiesterase7A,PDE7
SuppliedAs:
ThePDE7Ainhibitorscreeningassaykitcomesinaconvenient384-wellformat,includingpurifiedPDE7Aenzyme,fluorescentlylabeledPDE7Asubstrate(cAMP),bindingagent,andPDEassaybufferfor384enzymereactions.
Format:

COMPONENTS:

Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol
Storage/StABIlity:
Stableatleast6monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):

1.Malik,R.etal.(2008)Appl.Microbiol.Biotechnol.77(5):1167-1173.
2.Pekkinen,M.etal.(2008)Bone.43(1):84-91.

Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Phosphodiesterases
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
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