产品资料

Antibodies-Online/CAS9 (N-Term) antibody/ABIN2668367/100 μg

Antigen

Epitope

N-Term

Reactivity

Streptococcus pyogenes (S. pyogenes)

Host

Mouse

Clonality (Clone)

Conjugate

Un-conjugated

Application

Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

9 Western Blotting (WB)

4 ELISA

3 Fluorescence Microscopy
(FM)

3 Immunofluorescence (IF)

3 Immunoprecipitation (IP)

1 Chromatin
Immunoprecipitation
(ChIP)

1 Immunocytochemistry (ICC)

Options

Supplier

Supplier Product No.

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Immunogen	This antibody was raised against a recombinant protein within the N-terminal region of Streptococcus pyogene Cas9. This antibody should recognize Cas9 and dCas9 based on the antigen design.
Clone	7A9-3A3
Isotype	IgG1 kappa
Purification	Protein G Chromatography

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Background	Cas9 is a nuclease from Streptococcus pyogenes that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) Probable. In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
Molecular Weight	160 kDa

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Application Notes	Optimal working dilution should be determined by the investigator.
Restrictions	For Research Use only

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Concentration	1 μg/μL
Preservative	Sodium azide
Precaution of Use	This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice	Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Storage	-20 °C
Storage Comment	Antibodies in solution can be stored at -20 °C for 2 years.
Expiry Date	6 months

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Supplier Images	Cas9 antibody (mAb) tested by Immunoflourescence. HeLa cells were transiently transfe... Cas9 antibody (mAb) tested by Immunoprecipitation. 5 µg of Cas9 antibody (mAb) was us... Cas9 antibody (mAb) tested by Western blot. HEK293 cells were transiently transfected... Images for product: anti-CAS9 (N-Term) antibody Cas9 antibody (mAb) tested by Immunoflourescence. HeLa cells were transiently transfected with flag-tagged Cas9 for 48 hours and then stained. Top: Cas9 antibody (mAb) at a 0.5 µg/ml dilution. Bottom: Stained with Hoechst. Cas9 antibody (mAb) tested by Immunoprecipitation. 5 µg of Cas9 antibody (mAb) was used to immunoprecipitate Cas9 from 100 µg of whole cell extracts after 72 hours of transient transfection of HEK293 cells with flag-tagged Cas9. The immunoprecipitated protein was detected by Western blotting using Cas9 antibody (mAb) at a 0.5 µg/mL dilution. Cas9 antibody (mAb) tested by Western blot. HEK293 cells were transiently transfected with myc-tagged Cas9 (lane 2) or untransfected (lane 1), lysates prepared 40 hours post transfection, and probed with Cas9 antibody (mAb) at a 0.5 µg/ml dilution.